TABLE 1.

Plasmids used in this study

PlasmidRelevant characteristics and/or plasmid constructionaReference or source
pGEM-T Easy vectorCloning vector; AprPromega
pCP29E. coli-Flavobacterium shuttle plasmid; Apr (Cfxr Emr)28
pSCH03Promoter trap plasmid; promoterless gfpmut3 gene in pCP23; Apr (Tcr)12
PSCH143E. coli-Flavobacterium shuttle plasmid; Apr (Emr)This study
PSCH144Promoter trap plasmid; promoterless gfpmut3 gene in pSCH143; Apr (Emr)This study
pFj29ompA trapped in pSCH144; Apr (Emr)Unpublished data
OmpA.−54356-bp fragment of ompA promoter fused to gfpmut3; Apr (Emr)This study
OmpA.−48350-bp fragment of ompA promoter fused to gfpmut3; Apr (Emr)This study
OmpA.−32334-bp fragment of ompA promoter fused to gfpmut3; Apr (Emr)This study
OmpA.−5307-bp fragment of ompA promoter fused to gfpmut3; Apr (Emr)This study
OMPS-7Three-base change from TTT to AAA at positions −7 to −9; Apr (Emr)This study
OMPS-33Three-base change from TTG to AAC at positions −33 to −35; Apr (Emr)This study
OMPM-6ASingle-base change from G to A at position −6; Apr (Emr)This study
OMPM-6TSingle-base change from G to T at position −6; Apr (Emr)This study
OMPM-6CSingle-base change from G to C at position −6; Apr (Emr)This study
OMPM-7ASingle-base change from T to A at position −7; Apr (Emr)This study
OMPM-7GSingle-base change from T to G at position −7; Apr (Emr)This study
OMPM-7CSingle-base change from T to C at position −7; Apr (Emr)This study
OMPM-8ASingle-base change from T to A at position −8; Apr (Emr)This study
OMPM-8GSingle-base change from T to G at position −8; Apr (Emr)This study
OMPM-8CSingle-base change from T to C at position −8; Apr (Emr)This study
OMPM-9ASingle-base change from T to A at position −9; Apr (Emr)This study
OMPM-9GSingle-base change from T to G at position −9; Apr (Emr)This study
OMPM-9CSingle-base change from T to C at position −9; Apr (Emr)This study
OMPM-10ASingle-base change from T to A at position −10; Apr (Emr)This study
OMPM-10GSingle-base change from T to G at position −10; Apr (Emr)This study
OMPM-10CSingle-base change from T to C at position −10; Apr (Emr)This study
OMPM-11ASingle-base change from C to A at position −11; Apr (Emr)This study
OMPM-11GSingle-base change from C to G at position −11; Apr (Emr)This study
OMPM-11TSingle-base change from C to T at position −11; Apr (Emr)This study
OMPM-12TSingle-base change from A to T at position −12; Apr (Emr)This study
OMPM-12GSingle-base change from A to G at position −12; Apr (Emr)This study
OMPM-12CSingle-base change from A to C at position −12; Apr (Emr)This study
OMPM-13ASingle-base change from T to A at position −13; Apr (Emr)This study
OMPM-13GSingle-base change from T to G at position −13; Apr (Emr)This study
OMPM-13CSingle-base change from T to C at position −13; Apr (Emr)This study
OMPM-33ASingle-base change from G to A at position −33; Apr (Emr)This study
OMPM-33TSingle-base change from G to T at position −33; Apr (Emr)This study
OMPM-33CSingle-base change from G to C at position −33; Apr (Emr)This study
OMPM-34ASingle-base change from T to A at position −34; Apr (Emr)This study
OMPM-34GSingle-base change from T to G at position −34; Apr (Emr)This study
OMPM-34CSingle-base change from T to C at position −34; Apr (Emr)This study
OMPM-35ASingle-base change from T to A at position −35; Apr (Emr)This study
OMPM-35GSingle-base change from T to G at position −35; Apr (Emr)This study
OMPM-35CSingle-base change from T to C at position −35; Apr (Emr)This study
OMPM-36ASingle-base change from T to A at position −36; Apr (Emr)This study
OMPM-36GSingle-base change from T to G at position −36; Apr (Emr)This study
OMPM-36CSingle-base change from T to C at position −36; Apr (Emr)This study
OMPMS17Derivate of ompA promoter containing 17-bp spacerThis study
OMPMS18Derivate of ompA promoter containing 18-bp spacerThis study
OMPMS20Derivate of ompA promoter containing 20-bp spacerThis study
OMPMS21Derivate of ompA promoter containing 21-bp spacerThis study
OMPMS22Derivate of ompA promoter containing 22-bp spacerThis study
OMPMS23Derivate of ompA promoter containing 23-bp spacerThis study
  • a Antibiotic resistance phenotypes: ampicillin, Apr; tetracycline, Tcr; erythromycin, Emr; cefoxitin, Cfxr. Unless indicated otherwise, antibiotic resistance phenotypes are those expressed in E. coli. Antibiotic resistances in parentheses are those expressed in Flavobacterium strains but not in E. coli.