TABLE 2.

Genes differently expressed in the S. aureus hemB mutant for combined values over the whole time course (0 to 10 h)

Function group and ORF (N315)P value by t testaFold changebDescription
Adaptation to atypical conditions
    SA01470.003.16Capsular polysaccharide synthesis Cap5D
    SA01440.003.60Capsular polysaccharide synthesis Cap5A
    SA15490.002.64Hypothetical protein similar to serine proteinase Do heat shock protein HtrA
    SA01450.002.65Capsular polysaccharide synthesis Cap5B
    SA24940.003.05Cold shock protein
    SA10960.002.23Heat shock protein
    SA01460.002.89Capsular polysaccharide synthesis Cap8C
    SA23360.002.30ATP-dependent Clp proteinase chain ClpL
    SA01480.012.60Capsular polysaccharide synthesis Cap8E
    SA01500.032.04Capsular polysaccharide synthesis Cap5G
    SA01490.032.14Capsular polysaccharide synthesis Cap5F
Antibiotic production
    SA01730.012.19Hypothetical protein similar to surfactin synthetase
Cell wall
    SA16910.003.52Hypothetical protein similar to protein 1A/1B
    SA12830.002.14Penicillin-binding protein 2
    SA10240.002.32Penicillin-binding protein 1
Membrane bioenergetics (electron transport chain and ATP synthase)
    SA04110.005.00NADH dehydrogenase subunit
    SA02110.000.10Hypothetical protein similar to NADH-dependent dehydrogenase
    SA02100.000.13Hypothetical protein similar to NADH-dependent dehydrogenase
    SA03660.000.38Alkyl hydroperoxide reductase C
    SA09380.012.13Cytochrome d ubiquinol oxidase subunit II homologue
    SA11320.022.34Hypothetical protein similar to ferredoxin oxidoreductase beta subunit
    SA09370.022.14Cytochrome d ubiquinol oxidase subunit I homologue
    SA13110.022.12Hypothetical protein similar to thioredoxin reductase homologue
    SA13150.032.31Ferredoxin
Metabolism of amino acids and related molecules
    SA24280.004.96Arginine deiminase
    SA23410.000.381-Pyrroline-5-carboxylate dehydrogenase
    SA11650.000.39Threonine synthase
    SA11660.000.39Homoserine kinase homologue
    SA04190.012.13Cystathionine gamma-synthase
    SA04160.012.05Hypothetical protein similar to carboxylesterase
    SA24270.024.24Ornithine transcarbamoylase
    SA23890.032.06Truncated hypothetical protein similar to metalloproteinase Mpr precursor
Metabolism of carbohydrates and related molecules
    SA11420.005.46Aerobic glycerol-3-phosphate dehydrogenase
    SA09450.000.35Dihydrolipoamide S-acetyltransferase component of pyruvate dehydrogenase complex E2
    SA12450.000.202-Oxoglutarate dehydrogenase E1
    SA20080.003.40Alpha-acetolactate synthase
    SA07300.002.38Phosphoglycerate mutase
    SA11840.000.34Aconitate hydratase
    SA12440.000.26Dihydrolipoamide succinyltransferase
    SA11410.002.57Glycerol kinase
    SA07280.002.43Phosphoglycerate kinase
    SA20070.002.68Hypothetical protein similar to alpha-acetolactate decarboxylase
    SA07290.002.16Triosephosphate isomerase
    SA01820.000.36Hypothetical protein similar to indole-3-pyruvate decarboxylase
    SA15530.000.33Formyltetrahydrofolate synthetase
    SA10890.000.38SucD succinyl coenzyme A synthetase alpha chain
    SA23270.002.27Hypothetical protein similar to pyruvate oxidase
    SA15540.000.30Acetyl coenzyme A synthetase
    SA02320.0040.16l-Lactate dehydrogenase
    SA02190.012.22Formate acetyltransferase activating enzyme
    SA06540.022.40Fructose 1-phosphate kinase
    SA15170.020.39Isocitrate dehydrogenase
Metabolism of coenzymes and prosthetic groups
    SA14920.002.06Delta-aminolevulinic acid dehydratase
Metabolism of nucleotides and nucleic acids
    SA09230.000.22Phosphoribosylformylglycinamidine cycloligase PurM
    SA09240.000.23Phosphoribosylglycinamide formyltransferase
    SA09210.000.24Phosphoribosylformylglycinamidine synthetase PurL
    SA09220.000.26Phosphoribosylpyrophosphate amidotransferase PurF
    SA09160.000.28Hypothetical protein similar to phosphoribosylaminoimidazole carboxylase PurE
    SA09250.000.27Bifunctional purine biosynthesis PurH
    SA09200.000.34Phosphoribosylformylglycinamidine synthase II
    SA22970.002.63Hypothetical protein similar to GTP-pyrophosphokinase
    SA03730.000.32Xanthine phosphoribosyltransferase
    SA09180.010.39Phosphoribosylaminoimidazolesuccinocarboxamide synthetase homologue
Metabolism of phosphate
    SA23010.002.27Hypothetical protein similar to alkaline phosphatase
No similarity
    SA22210.003.60Hypothetical protein
    SA14760.005.66Hypothetical protein
    SA20110.004.10Hypothetical protein
    SA22720.002.05Hypothetical protein
    SA17740.002.29(Bacteriophage phiN315) hypothetical protein
    SA05360.003.20Hypothetical protein
    SA20910.000.26Hypothetical protein
    SA17730.002.26(Bacteriophage phiN315) hypothetical protein
    SA17720.002.48(Bacteriophage phiN315) hypothetical protein
    SA17030.003.22Hypothetical protein
    SA05350.012.47Hypothetical protein
    SA08850.022.12Hypothetical protein
    SA21130.032.25Hypothetical protein
    SAS0140.032.22Hypothetical protein
    SA22680.042.16Hypothetical protein
Pathogenic factors (toxins and colonization factors)
    SA05870.000.13Lipoprotein streptococcal adhesin PsaA
    SA24300.002.37Zinc metalloproteinase aureolysin
    SA22060.000.29Immunoglobulin G-binding protein SBI
    SA03900.003.81(Pathogenicity island SaPIn2) 14
    SA20970.012.68Hypothetical protein similar to secretory antigen precursor SsaA
    SA07420.012.14Fibrinogen-binding protein A
    SA23560.022.12Immunodominant antigen A
Phage-related functions
    SA17960.006.74(Bacteriophage phiN315) hypothetical protein
    SA17820.002.86(Bacteriophage phiN315) hypothetical protein
    SA17850.002.04(Bacteriophage phiN315) hypothetical protein
    SA17830.012.38(Bacteriophage phiN315) hypothetical protein
    SA17880.022.03(Bacteriophage phiN315) hypothetical protein
    SA17750.022.31(Bacteriophage phiN315) hypothetical protein
    SA17970.022.12(Bacteriophage phiN315) hypothetical protein
Protein folding
    SA16590.002.89Peptidyl-prolyl cis/trans isomerase homologue
RNA modification
    SA17130.022.10RNA methyltransferase homologue
    SA18850.022.13Hypothetical protein similar to RNA helicase
RNA synthesis
    SA24240.009.18Hypothetical protein similar to transcription regulator Crp/Fnr family protein
    SA20920.000.20Hypothetical protein similar to transcription regulator
    SA19490.004.15Lytic regulatory protein truncated with Tn554
    SA19560.003.35Lytic regulatory protein truncated with Tn554
    SA17000.004.09Two-component response regulator
    SA24290.003.61Hypothetical protein similar to arginine repressor
    SA01080.000.30Staphylococcal accessory regulator A
    SA22960.002.71Hypothetical protein similar to transcriptional regulator MerR family
    SA21030.002.20Hypothetical protein similar to divergon expression attenuator LytR
    SA11390.002.11Glycerol uptake operon regulatory protein
    SA06530.002.82Hypothetical protein similar to repressor of fructose operon
    SA19610.002.26Hypothetical protein similar to transcription antiterminator BglG family
    SA24180.012.18Hypothetical protein similar to response regulator
    SA01870.010.39Hypothetical protein similar to transcription regulator
    SA22950.032.65Gluconate operon transcriptional repressor
    SA22870.032.06Staphylococcal accessory regulator A
Sensors (signal transduction)
    SA17010.004.09Two-component sensor histidine kinase
    SA16530.002.03Signal transduction protein
    SA24170.012.15Hypothetical protein similar to sensor histidine kinase
Similar to unknown proteins
    SA01750.005.39Conserved hypothetical protein
    SA17020.003.31Conserved hypothetical protein
    SA04120.005.20Conserved hypothetical protein
    SA02130.000.23Conserved hypothetical protein
    SA22200.003.26Conserved hypothetical protein
    SA04130.002.83Conserved hypothetical protein
    SA02120.000.14Conserved hypothetical protein
    SA05880.000.13Conserved hypothetical protein
    SA23290.003.96Conserved hypothetical protein
    SA17120.003.19Conserved hypothetical protein
    SA09080.003.66Conserved hypothetical protein
    SA21460.002.72TcaA protein
    SA09190.000.31Conserved hypothetical protein
    SA07250.002.75Conserved hypothetical protein
    SA10210.002.33Conserved hypothetical protein
    SA10310.002.67Conserved hypothetical protein
    SA10320.002.17Conserved hypothetical protein
    SA08240.002.29Conserved hypothetical protein
    SA14330.010.37Conserved hypothetical protein
    SA03010.010.40Conserved hypothetical protein
    SA09620.012.08Conserved hypothetical protein
    SA10330.022.38Conserved hypothetical protein
    SA24810.022.23Conserved hypothetical protein
    SA04150.022.16Conserved hypothetical protein
    SA22980.032.01Conserved hypothetical protein
    SA01740.042.01Conserved hypothetical protein
    SA10710.042.02Conserved hypothetical protein
Transport/binding proteins and lipoproteins
    SA24260.0016.29Arginine/ornithine antiporter
    SA05410.004.96Hypothetical protein similar to amino acid transporter
    SA11400.005.59Glycerol uptake facilitator
    SA19600.003.85PTS mannitol-specific IIBC component
    SA02930.003.77Hypothetical protein similar to formate transporter NirC
    SA21560.003.72Permease LctP homologue
    SA05890.000.11Hypothetical protein similar to ABC transporter ATP-binding protein
    SA23030.000.21Hypothetical protein similar to membrane-spanning protein
    SA02080.000.13Maltose/maltodextrin transport permease homologue
    SA23020.000.31Hypothetical protein similar to ABC transporter
    SA01110.000.38Lipoprotein
    SA08480.000.35Oligopeptide transport system ATP-binding OppF homologue
    SA21670.002.69PTS sucrose-specific component
    SA02070.000.21Hypothetical protein similar to maltose/maltodextrin-binding protein
    SA08490.000.35Hypothetical protein similar to binding protein OppA
    SA04320.002.52PTS enzyme II, phosphoenolpyruvate dependent, trehalose specific
    SA02090.000.20Maltose/maltodextrin transport permease homologue
    SA02060.000.20Multiple sugar-binding transport protein
    SA06400.002.01Hypothetical protein similar to ABC transporter required for expression of cytochrome bd
    SA01720.002.19Hypothetical protein similar to membrane protein LmrP
    SA01860.000.38Hypothetical protein similar to phosphotransferase enzyme II
    SA06390.012.04Hypothetical protein similar to ABC transporter required for expression of cytochrome bd
    SA05310.012.20Proline/betaine transporter homologue
    SA04170.012.15Hypothetical protein similar to sodium-dependent transporter
    SA06550.012.25Fructose-specific permease
    SA03740.010.39Xanthine permease
    SA20530.012.06Glucose uptake protein homologue
    SA24340.022.31Fructose phosphotransferase system enzyme homologue
  • a Only ORFs with a P value by t test of <0.05 have been listed.

  • b Only ORFs with a fold change of <0.4 or ≥2.0 for the hemB mutant versus the parent strain have been listed.