TABLE 1.

Characteristics of the streptococcal strains used in this study

StrainaRelevant characteristicsSource or referenceb
S. pneumoniae
    Unencapsulated strains
        R6D39 derivative; lytA+32
        M11lytA+23
        M31ΔlytA61
        M31BM31 but lytB::ermC; Emr24
        M31BCM31B but lytC::tet; Emr TcrB. de las Rivas; CSIC
        M31BCDM31 but lytB::neo lytC::tet pce::ermC; Kanr Tcr EmrB. de las Rivas
        M32ΔlytA3247
        R6BR6 but lytB::ermC; Emr24
        R6BCR6B but lytC::tet; Emr TcrB. de las Rivas
        R6CR6 but lytC::ermC; Emr52
        R6DR6 but pce::ermC; Emr13
        R924R6 but lytA::kan by mariner mutagenesisc; Kanr51
        R1582R6 but cbpD::spc by mariner mutagenesisc; SpcrJ. P. Claverys; CNRS
        P040R6 (pMV158GFP) by transformation with plasmid DNA; TcrThis study
        P042R6 but lytA::kan lytC::tet; R924 transformed by chromosomal DNA from R6DC; Kanr TcrThis study
        P046R6 but lytA::kan lytC::ermC; R924 transformed by chromosomal DNA R6C; Kanr EmrThis study
        P049R6 but pce::ermC (pMV158GFP); R6D transformed by pMV158GFP; Emr TcrThis study
        P050R6 but lytA::kan lytC::ermC (pMV158GFP); P046 transformed by pMV158GFP; Kanr Emr TcrThis study
        P064R6 but cbpA::spc by mariner mutagenesisd; SpcrThis study
        P065R6 but pcpA::cat by mariner mutagenesisd; CmrThis study
        P066R6 but pspA::kan by mariner mutagenesisc; KanrThis study
        ST344lytA+4
    Encapsulated strains
        13868S1+52
        P005M11 but S1+eD. Llull; CSIC
        D39S2+G. Pozzi; US
        P006M11 but S2+eD. Llull
        TIGR4S4+67
        P008M11 but S4+eD. Llull
        Spain6B-2S6B+A. Fenoll; ISCIII
        P010M11 but S6B+eD. Llull
        SSISP9V/1S9V+Staten Seruminstitut
        P019M11 but S9V+eD. Llull
        Spain14-5S14+A. Fenoll
        P021M11 but S14+eD. Llull
        G54S19F+19
        P015M11, but S19F+eD. Llull
        SSISP23F/1S23F+Staten Seruminstitut
        P022M11 but S23F+eD. Llull
        7077/39S37+42
        P018M11 but S37+eD. Llull
Mitis group streptococci
    Streptococcus mitisTNCTC
    Streptococcus oralisTCECT
    Streptococcus gordoniiTCECT
    Streptococcus pseudopneumoniaeTCCUG
  • a ST, sequence type; superscript T, type strain.

  • b CSIC, Consejo Superior de Investigaciones Científicas, Spain; CNRS, Centre National de la Recherche Scientifique, France; US, Università di Siena, Italy; ISCIII, Instituto de Salud Carlos III, Spain; NCTC, National Collection of Type Cultures; CECT, Colección Española de Cultivos Tipo; CCUG, Culture Collection, University of Göteborg, Sweden.

  • c Cotranscribed orientation of the antibiotic resistance cassette of the minitransposon with respect to the targeted gene.

  • d Antitranscribed orientation of the antibiotic resistance cassette of the minitransposon with respect to the targeted gene.

  • e S1+ to S37+ indicate that the M11 transformant synthesizes a capsular polysaccharide of the corresponding serotype.