Bacterial strains and plasmids

Strain or plasmidDescription and/or relevant genotypeaSource or reference
    pBluescript II.SKApr, high copy numberStratagene
    pBC.SKCmr, high copy numberStratagene
    pKNG101R6K-derived suicide vector, Smr17
    pSK.DisApSK with disA RBS and coding regionThis study
    pBC.DisApBC.SK with disA RBS and coding regionThis study
E. coli strains
    XL1-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-pro) [F′ proAB lacIqZΔM15 Tn10]Stratagene
    SM10 λpirthi thr leu tonA lacY supE recA RP4-2-Tc::Mu Kmr λpirV. deLorenzo
P. mirabilis strains
    PM7002Wild typeATCC
    PM437speA::mini-Tn5 lacZ127
    PM539Nonswarming; putrescine producer26
    LS1PM437 disA1::mini-Tn5CmThis study
    LS2PM437 disA2::SmrThis study
    LS3PM7002 disA2::SmrThis study
    LS10PM7002 disA3::mini-Tn5 lacZ1This study
  • a RBS, ribosome binding site.