TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsSource or reference
S. mutans strains
    GS5wta; Ems KmsSUNYaBb
    UA159wt; Ems KmsJ. Ferretti, OU-ACGTc
    ΔbipGS5::pMM503; Bip EmrThis study
    ΔsmbGGS5::pMM504; SmbG EmrThis study
    H1GS5 with transposon Tn916 inserted at the upstream of Smb operon; Tetr24
    GS5 ΔcomCGS5::pAYCA1301; ComC Emr25
    UA159 ΔcomCUA159::pAYCA1301; ComC EmrThis study
E. coli DH5aCloning hostGIBCO BRL
Plasmids
    pCR2.1Cloning vector; AmprInvitrogen
    pUC19Cloning vectorPromega
    pMM501pUC19 harboring a bip gene; AmprThis study
    pMM502pUC19 harboring an smbG gene; AmprThis study
    pMM503pUC19 harboring an inactivated bip gene with an Emr cassette; Emr AmprThis study
    pMM504pUC19 harboring an inactivated smbG gene with an Emr cassette; Emr AmprThis study
  • a wt, wild type.

  • b SUNYaB, culture collection at the Department of Oral Biology, State University of New York, Buffalo, N.Y.

  • c OU-ACGT, University of Oklahoma Advanced Center for Genome Technology.