TABLE 1.

Essential genes for growth on glycerol minimal mediuma

GroupGene (Blattner no.)GroupGene (Blattner no.)
Amino acid metabolismargA (b2818)guaA (b2507)
argE (b3957)guaB (b2508)
argH (b3960)purA (b4177)
aroA (b0908)purC (b2476)
aroB (b3389)purD (b4005)
aroC (b2329)purE (b0523)
aroD (b1693)purF (b2312)
aroE (b3281)purH (b4006)
cysC (b2750)purK (b0522)
cysD (b2752)purL (b2557)
cysE (b3607)purM (b2499)
cysH (b2762)pyrB (b4245)
cysI (b2763)pyrC (b1062)
cysJ (b2764)pyrD (b0945)
cysK (b2414)pyrE (b3642)
cysN (b2751)pyrF (b1281)
cysP (b2425)thyA (b2827)
[cysQ (b4214)]
glyA (b2551)Cofactor productionfolB (b3058)
hisA (b2024)folP (b3177)
hisB (b2022)iscC (b2530)
hisC (b2021)nadA (b0750)
hisD (b2020)nadB (b2574)
hisE (b2025)nadC (b0109)
hisG (b2019)panB (b0134)
hisH (b2023)panC (b0133)
hisI (b2026)panD (b0131)
ilvA (b3772)pdxA (b0052)
ilvB (b3671)pdxB (b2320)
ilvC (b3774)pdxH (b1638)
ilvD (b3771)pdxJ (b2564)
leuA (b0074)ubiG (b2232)
leuB (b0073)ubiH (b2907)
leuC (b0072)
leuD (b0071)Regulatory proteinscysB (b1275)
lysA (b2838)fruR (b0080)
metA (b4013)[leuL (b0075)]
metB (b3939)metR (b3828)
metC (b3008)
metF (b3941)Transportcrr (b2417)
metL (b3940)cysA (b2422)
pabA (b3360)cysU (b2424)
pabB (b1812)[fes (b0585)]
pheA (b2599)ptsI (b2416)
proA (b0243)
proB (b0242)OthersatpA (b3734)
proC (b0386)atpB (b3738)
serA (b2913)atpC (b3731)
serB (b4388)atpF (b3736)
serC (b0907)atpG (b3733)
thrA (b0002)atpH (b3735)
thrB (b0003)glmM (b3176)
thrC (b0004)glnA (b3870)
trpA (b1260)glpD (b3426)
trpB (b1261)glpK (b3926)
trpC (b1262)gltA (b0720)
trpD (b1263)icd (b1136)
trpE (b1264)ppc (b3956)
tyrA (b2600)[prfB (b2891)]
[rpsU (b3065)]
Purine and pyrimidine biosynthesiscarA (b0032)[yhhK (b3459)]
carB (b0033)[yjhS (b4309)]
  • a The 119 genes identified as being required for growth on glycerol M9 minimal medium are divided into six groups based on shared annotations and/or properties. The 26 genes that appear in bold-face represent genes that were not predicted to be essential by the model and were not associated with essential reactions (described in further detail in the text and Table 2). The seven genes in brackets are the essential genes identified in this study that do not appear in the model.