TABLE 2.

Plasmids used in this study

PlasmidGene constructionReference or source
pNZ8048nisA promoter + NcoI site + multiple cloning site + terminator, pSH71 replicon, cam, translational fusion vector for nisin-controlled expression9
pNZ9530nisR and nisK (both expressed from rep promoter), pAMβ1 replicon, ery8
pQU1100fsrB associated with a nisA promoter upstream and a transcriptional terminator downstream cloned into a pGEM-T vectorThis study
pQU2100Chimera of pQU1100 and pNZ9530This study
pQU2101pQU2100 derivative carrying fsrB with the deletion of 568ATGThis study
pQU2102pQU2100 derivative carrying fsrB with a mutation of 568ATG to 568GCAThis study
pQU2103pQU2100 derivative carrying fsrB with a mutation of 556GGA to 556AAAThis study
pQU2104pQU2100 derivative carrying fsrB with the deletion of 548TThis study
pQU2200pQU2100 derivative carrying fsrB′ that is a 3′-end truncation of fsrB, lacking in the fsrD regionThis study
pQU2231pQU2200 derivative carrying fsrB′ coding for R66A mutationThis study
pQU2241pQU2200 derivative carrying fsrB′ coding for G71A mutationThis study
pQU2251pQU2200 derivative carrying fsrB′ coding for H73F mutationThis study
pQU2261pQU2200 derivative carrying fsrB′ coding for C80A mutationThis study
pQU2271pQU2200 derivative carrying fsrB′ coding for P123A and P130A mutationsThis study
pQU2281pQU2200 derivative carrying fsrB′ coding for K140A and K141A mutationsThis study
pQU2300fsrD translationally fused to pNZ8048This study