TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionSource or reference
B. subtilis
    EB6 hisA1 argC4 metC3 L5087 (2)
    EB240 hisA1 argC4 metC3 amyE::xylR PxylA tagD cat86 tagD::Specr 2
    EB633 hisA1 argC4 metC3 amyE::xylR PxylA tagB cat86 tagB::Specr 4
    EB669 hisA1 argC4 metC3 amyE::xylR PxylA tagF cat86 tagF::Specr 4
    EB892EB6 transformed with pRBtagBgfp 5
    EB1451 hisA1 argC4 metC3 tagO::Ermr This study
    EB1453 hisA1 argC4 metC3 amyE::xylR PxylA tagB cat86 tagB::Specr tagO::Ermr This study
    EB1559 hisA1 argC4 metC3 amyE::xylR PxylA tagD cat86 tagD::Specr tagO::Ermr This study
    EB1560 hisA1 argC4 metC3 amyE::xylR PxylA tagF cat86 tagF::SpecR tagO::Ermr This study
Plasmids
    pMUTIN4IPTGa-inducible integration vector, source of Ermr cassette 17
    pUS19pUC19 derivative containing Specr cassette 1
  • a IPTG, isopropyl-β-d-thiogalactopyranoside.