TABLE 1.

Metal dependence of recombinantly expressed carboxysomal carbonic anhydrase rCsoSCA from H. neapolitanus

Metal added (1 mM)Relative activity (%)a
None100
Zn2+92.8
Co2+101.8
Fe2+98.1
Mg2+98.4
Chelex-treated buffer28.4
Chelex-treated buffer + Zn2+84.6
Chelex-treated buffer + Co2+25.9
Chelex-treated buffer + Fe2+33.4
Chelex-treated buffer + Mg2+26.7
  • a CO2 hydration by rCsoSCA (0.5 μM) was measured by stopped-flow spectrophotometry at pH 8 (buffer/indicator pair, TAPS/m-cresol purple). Values are averages of three determinations with maximum variations of less than 12%.