TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeReference or source
E. coli strains
    CFT073Wild-type pyelonephritis isolate (O6:K2:H1)44
    CFT073 fim L-ONCFT073 fim IE phase locked; constitutive type 1 fimbrial expression22
    CFT073 fim L-OFFCFT073 fim IE phase locked; no type 1 fimbrial expression22
    fim L-ON ΔfimAICDFGHCFT073 fim L-ON with deletion of fimAICDFGHThis study
    UPEC76CFT073 Nalr derivative; both pap operons disrupted45
    Δfim papUPEC76 fimAICDFGH::aphA (Kanr)55
    ΔflhDCCFT073 flhDC::aphA (Kanr)This study
    ΔflhCCFT073 flhC::aphA (Kanr)This study
    ΔfliACFT073 fliA::aphA (Kanr)32
    ΔflgMCFT073 flgM::aphA (Kanr)32
    ΔfliCCFT073 fliC::aphA (Kanr)32
    ΔcheYCFT073 cheY::aphA (Kanr)32
    F11Wild-type cystitis isolate (O6:H31)58
    F11 fim L-ONF11 fim IE phase locked; constitutive type 1 fimbrial expression56
    F11 fim L-OFFF11 fim IE phase locked; no type 1 fimbrial expression56
    K-12Wild-type K-12 strain MG1655Gift from R. Welch (6)
    K-12 fim L-ONK-12 strain MG1655 fim IE phase locked; constitutive type 1 fimbrial expression (AAEC554)Gift from I. Blomfield (40)
    K-12 fim L-OFFK-12 strain MG1655 fim IE phase locked; no type 1 fimbrial expression (AAEC358)Gift from I. Blomfield (40)
Plasmids
    pBAD/Myc-HisAArabinose-inducible expression vector (Ampr)Invitrogen
    pBAD-flhDCpBAD/Myc-HisA digested with NcoI and PmeI replacing Myc-His site with flhDC (Ampr)This study