TABLE 2.

Oligonucleotides used in this study

PrimerSequence (5′-3′)Description
flhDCP1 (forward)TAATGCATACCTCCGAGTTGCTGAAACACATTTATGACATATGGGAATTAGCCATGGTCCConstruction of isogenic flhDC mutant
flhDCP2 (reverse)AAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCAGTGTAGGCTGGAGCTGCTTCConstruction of isogenic flhDC mutant
flhCP1 (forward)AAAGCATTGTTCAGGAAGCGCGGGATATTCAGCTGGCAATGTGTAGGCTGGAGCTGCTTCConstruction of isogenic flhC mutant
flhCP2 (reverse)CTGTACTCTCTGTTCATCCAGCAGTTGTGGGATAATATCGATGGGAATTAGCCATGGTCCConstruction of isogenic flhC mutant
fimA-HP1 (forward)GGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTGACAAAGTGTAGGCTGGAGCTGCTTCConstruction of isogenic fim L-ON fimAICDFGH mutant
fimA-HP2 (reverse)GTGCAGGTTTTAGCTTCAGGTAATATTGCGTACCTGCATTATGGGAATTAGCCATGGTCCConstruction of isogenic fim L-ON fimAICDFGH mutant
flhDCBAD (forward)CTGATTCCATGGGAATAATGCATACCTCCGAGTTGConstruction of pBAD-flhDC
flhDCBAD (reverse)ACTGGTGTTTAAACTTAAACAGCCTGTACTCTCTGTTCConstruction of pBAD-flhDC
gapA (forward)CGTTAAAGGCGCTAACTTCGqPCR
gapA (reverse)ACGGTGGTCATCAGACCTTCqPCR
fliC (forward)ACAGCCTCTCGCTGATCACTCAAAqPCR
fliC (reverse)GCGCTGTTAATACGCAAGCCAGAAqPCR
fimA (forward)GTTGGCTTTGGATGGTGCGTCATTqPCR
fimA (reverse)GGTTGCGGCACCAATGGCATAATAqPCR