TABLE 4.

Enzymatic activities in wild-type and mutant strains

Strain (description)Activity of the indicated enzyme (nmol mg of protein−1 min−1)a
Thr ammonia-lyaseSer ammonia-lyaseCitramalate synthaseIsopropyl malate synthase
DL1 (wild type)189 ± 4641 ± 6.27.4 ± 0.28.3 ± 0.4
DLCR5 (tdcB::Knr)NDND5.1 ± 0.28.5 ± 0.7
DLCR6 (cimA::Gmr)217 ± 240.4 ± 0.39.2 ± 0.3
DLCR7 (tdcB::Knr cimA::Gmr)ND0.4 ± 0.418.1 ± 1.3
  • a Measurements are averages and standard deviations of triplicate assays. Soluble extracts were prepared from early-stationary-phase NBAF cultures. ND, not detected; −, not assayed.