TABLE 1.

Bacterial strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant characteristic(s) or sequence (5′→3′)Source or reference
Serratia marcescens strains
    CH-1Clinical isolate 15
    CH-1ΔA rssA knockout mutant 15
Plasmids
    pGEXExpression vector, GST tagPharmacia Biotech
    pGST-BpGEX::rssBSm ; Ampr Smr This study
    pGST-B(D51E)pGEX::rssBSm (D51E); Ampr Smr This study
    pBG300pACYC184 (PflhDCSm ::luxCDABE); Cmr 26
    pBG301pACYC184 (P1flhDCSm ::luxCDABE); Smr Cmr This study
    pBG302pACYC184 (P2flhDCSm ::luxCDABE); Smr Cmr This study
    pBGpACYC184 (promoterless luxCDABE); Smr Cmr This study
Primers
    FlhD-PFCAGCCTCAGGCGGAGGGThis study
    FlhD-PRATTCCCCATCCCGACAGAGCTAThis study
    F0-FTGCTAATGGTTCAGGGGThis study
    F0-RDIG-ATTCCCCATCCCGACAGACTAThis study
    Fa-FCAGCCTCAGGCGGAGGGThis study
    Fa-RDIG-CTGAACCATTAGCACACAThis study
    Fb-FCAGCCTCAGGCGGAGGGThis study
    Fb-RTTGCTACGAGCGTAAACCAAThis study
    FlhD-PEFAM-CGTACCCATATTCCCCATCCCThis study
    flhD-SFTGTCGGGATGGGGAATATGGThis study
    flhD-SRCGATAGCTCTTGCAGTAAATGGThis study