TABLE 2.

Comparison of DH10B, MG1655, and W3110 sequences

Region affectedaTypebPolymorphism inc:DH10B correspondenced
MG1655W3110
alsKInsertionwtIS5MG1655
dcuCInsertionwtIS5MG1655
gatAInsertionwtIS5MG1655
rcsCInsertionwtIS2MG1655
tdcDInsertionwtIS5MG1655
tnaBInsertionwtIS5MG1655
flhD promoterInsertionIS1IS5W3110
csgC-IG-ymdAInsertionwtIS2MG1655
lrhA-IG-yfhQInsertionwtIS1MG1655
tnaC-IG-tnaAInsertionwtIS5MG1655
ychE-IG-oppAInsertionwtIS2IS1A.2
yieE-IG-yidZInsertionwtIS2MG1655
eutA-IG-eutBInsertionCPZ-55wtMG1655
acnAMissenseA (A522)G (G522)MG1655
crpMissenseC (T29)A (K29)MG1655
intQMissenseT (F274)C (L274)IS2
ycdTMissenseC (A130)T (V130)IS2
rpoSNonsenseC (Q33)T (STOP33)MG1655
yedJSilentC (V219)T (V219)MG1655
rrlESNPA2256G2256MG1655
dcuAFrameshiftwtTT after nt 182MG1655
  • a Mutations within genes are indicated by the gene name only. Mutations in intergenic (IG) regions are indicated along with flanking gene names.

  • b Type of mutation in W3110 relative to MG1655.

  • c SNPs in conserved domain sequences are indicated by the base present and the corresponding amino acid in parentheses. For rrlE, the base change and position within the gene are given. For dcuA, TT is inserted after nucleotide 182 in the gene. CPZ-55 is a prophage. wt, wild type.

  • d DH10B correspondence with MG1655 or W3110 is indicated. Where DH10B differs from both, the associated change is noted.