TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescription or relevant genotypeReference or source
E. coli strains
    JKD7-1W3110 ftsZ::kan recA5615
    W3110Wild-type strain
    AND101W3110 Δ(λattL-lon)::bla lacIq P208-ftsZ-gfpThis study
Plasmids
    pKD4ftsZ on pGB25
    pGAD424Yeast two-hybrid vectorClontech
    pZ-F268EpKD4 with F268 of ftsZ mutated to EThis study
    pZ-F268ApKD4 with F268 of ftsZ mutated to AThis study
    pZ-F269ApKD4 with F269 of ftsZ mutated to AThis study
    pZ-P300ApKD4 with P300 of ftsZ mutated to AThis study
    pZ-C149ApKD4 with C149 of ftsZ mutated to AThis study
    pZ-R31ApKD4 with R31 of ftsZ mutated to AThis study
    pAND110ftsZ(F268A) cloned into pGAD424This study
    pAND111ftsZ(F269A) cloned into pGAD424This study
    pAND112ftsZ(P300A) cloned into pGAD424This study
    pACT3Expression vector20
    pA3Chromosomal sulA cloned into pACT3 cut with XbaI and HindIIIThis study
    pKD126ftsZ cloned in pJF118EH44
    pJC93malE-sulA fusion cloned into pBAD1844
    pJC94malE-sulA10 fusion cloned into pBAD1844
    pZAQpBR322 derivative carrying ftsQ, ftsA, and ftsZ5
    pBR322
    pBEF0pGB2 derivative carrying ftsA, ftsZ, and envA5
    pGB2Low-copy-number vector with pSC101 origin of replication13
    pSEB160ftsZ gene cloned downstream of the arabinose promoter on pBAD18S. Pichoff and J. Lutkenhaus, unpublished data
    pAND403GFP-sulA cloned into pBAD18 cut with HindIII and XbaIThis study