TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionaReference or source
Strains
    B. mallei ATCC 23344Wild-type pathogen, Pmr Kms Gms Zeos 49
    E. coli TOP10General cloning strainInvitrogen
    E. coli S17-1Tra+ Smr Pms 57
    B. mallei DD3008Capsule-deficient mutant 14
    B. mallei RD01T3SSAP-deficient mutant 64
B. mallei derivativesb
    GRS 23344ΔsacB sucrose-resistant derivativeThis study
    IlvBMA1848 inactivated with pCRXL-ilvI; ilv auxotrophThis study
    GspDBMA2786 inactivated with pLCL9; type II secretion system mutantThis study
    A0235BMA0235 inactivated with pCRXL-A0235This study
    AA1900BMAA1900 inactivated with pCRXL-AA1900This study
    AA1204BMAA1204 inactivated with pCRXL-AA1204This study
    AA1013BMAA1013 inactivated with pCRXL-AA1013This study
    A1123BMA1123 inactivated with pCRXL-A1123This study
    AA1111BMAA1111 inactivated with pCRXL-dictyThis study
    AA1785BMAA1785 inactivated with pCRXL-chitinThis study
    A0847BMA0847 inactivated with pCRXL-A0847This study
    Δ1517BMAA1517 deletion in GRS 23344This study
    Δ1621BMAA1621 deletion in GRS 23344This study
Plasmids
    pCR-XL-TOPOCloning vector, Kmr Zeor Invitrogen
    pCR2.1-TOPOCloning vector, Kmr Ampr Invitrogen
    pGRV2Gene replacement suicide vector, Gmr 63
    pDD159 sacB gene deletion alleleThis study
    pCRXL1517SOESpliced flanking regions of BMAA1517This study
    pCRXL1621SOESpliced flanking regions of BMAA1621This study
    pGRV1517SOEBamHI-XbaI fragment from pCRXL1517SOEThis study
    pGRV1621SOESacI-BamHI fragment from pCRXL1621SOEThis study
  • a Pm, polymyxin B; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Zeo, zeocin; Amp, ampicillin.

  • b A gene was inactivated by insertion of a pCR-XL-TOPO vector containing a 400-to 900-bp PCR-amplified internal fragment of the gene.