TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or relevant characteristic(s)Source or reference
Bacterial strains
    E. coli XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q lacZΔM15 Tn10(Tcr)]Laboratory stock
    S. aureus RN4220 (NCTC 8325)Restriction-minus derivative of 8325-4, primary recipient for propagation of E. coli plasmids, contains an 11-bp deletion in the rsbU gene 32
    S. aureus SH10008325-4 carrying an intact rsbU geneS. Foster (28)
    S. aureus SH1000 ΔarcR SH1000 in which the arcR gene was deleted and replaced by the Ωkm-2 kanamycin cassetteThis study
Plasmids
    pUC18Cloning vector; lacZ Apr MBI/Fermentas
    PMUTIN-4pUC18-based suicidal vector for gram-positive bacteria; Pspac-lacZ PpenP-lacI Apr Emr 51
    pAUL-ATemperature-sensitive shuttle vector; lacZ Emr 47
    pBR322::Ωkm-2pBR322 carrying the Ωkm-2 cassette; Apr Kmr 43
    pGEM-T Easy vectorAn EcoRV-linearized vector with 3′-added T bases at both endsPromega
    pET28a(+)A T7-based protein expression vector carrying an N-terminal His tag/thrombin tagNovagen