TABLE 2.

Oligonucleotide primers used for construction by PCR of DNA probes

PrimerPrimer sequenceaDescription
arcR_FOR5′-CATATGACAGAAAACTTTATTTTGGG-3′Amplification of arcR gene
arcR_REV5′-GGATCCTTAAACACATACATCATTG-3′Amplification of arcR gene
arcA_for5′-GACCCAAAATACCTTTATTTATG-3′Amplification of 233-bp probe in arcA regulatory region
arcA_rev5′-CCAGGACGCTTAAGTAACAC-3′Amplification of 233-bp probe in arcA regulatory region
lctE_for5′-CACTGGCGAAGTACGAAGAC-3′Amplification of 210-bp probe in lctE regulatory region
lctE_rev5′-AAAGGTCATGTGTCATCCGC-3′Amplification of 210-bp probe in lctE regulatory region
adh1_for5′-TGTCTTAGATTGATTGGGAG-3′Amplification of 215-bp probe in adh1 regulatory region
adh1_rev5′-GACATAATCGATATGCTAACG-3′Amplification of 215-bp probe in adh1 regulatory region
lukM_for5′-ATTAATGACTTTGTACACAC-3′Amplification of 211-bp probe in lukM regulatory region
lukM_rev5′-GCACATGATAATGATGACGC-3′Amplification of 211-bp probe in lukM regulatory region
srrA_for5′-GTCATTTAGCAGAACATGGG-3′Amplification of 157-bp probe in srrAB regulatory region
srrA_for5′-ACAGGTCATACCTCCCACAC-3′Amplification of 157-bp probe in srrAB regulatory region
nrdD_for5′-ACATGTCGAAATGACGGACG-3′Amplification of 221-bp probe in nrdD regulatory region
nrdD_rev5′-AACCCGTTAATGCTTCTTCG-3′Amplification of 221-bp probe in nrdD regulatory region
arcA mut_for5′-TATGTGAATATAATGGGATGTAAGCGTTTGAAG-3′Mutagenesis
arcA mut_rev5′-AACGCTTACATCCCATTATATTCACATAAAG-3′Mutagenesis
  • a The mismatched bases for the mutations of primers used for mutagenesis are underlined.