TABLE 2.

Effect of various carbon sources and regulatory mutations on the expression of the kdgN-uidA fusiona

Mutated gene(s)Carbon sourceActivityb
NoneGlycerol100 ± 11
NoneGlycerol plus galacturonate188 ± 11
kdgK Glycerol plus galacturonate640 ± 6
kdgR Glycerol527 ± 33
pecS Glycerol40 ± 3
kdgR and pecS Glycerol98 ± 9
NoneGlucose22 ± 3
crp Glucose5 ± 1
crp and pecS Glucose3 ± 0.5
ompR Glycerol19 ± 1
ompR and pecS Glycerol33 ± 1
ompR and kdgR Glycerol78 ± 7
hns Glycerol58 ± 4
  • a Bacteria were grown in M63 medium with the indicated carbon source at 0.2%.

  • b Activities are expressed as percentages of the activity obtained for the wild type with glycerol as the sole carbon source.