TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference
Y. enterocolitica strains
    YVM150JB580 ΔyenR (R M+)23
    YVM927JB580 ΔyenR (R M+) ΔrovA10
    YVM1251JB580 ΔyenR (R M+) pYV ΔlacZ ΔaraGFB::[φ(rovAYent-lacZY)]This study
    YVM1252JB580 ΔyenR (R M+) pYV ΔlacZ ΔaraGFB::[φ(rovAYent-lacZY)] ΔleuOThis study
    YVM1253JB580 ΔyenR (R M+) ΔrpoSThis study
    YVM1254JB580 ΔyenR (R M+) ΔrovMThis study
    TM102JB580 ΔyenR (R M+) pYV ΔlacZ ΔaraGFB::[φ(rovAYent-lacZY)] leuO::miniTn5 KnrThis study
Y. pestis strains
    YP6CO92 pCD17
    YP12CO92 pCD1 ΔrovA3
E. coli strains
    MC4100F araD139 Δ(lacIOPZYA)U169 rpsL thiA14
    VM1303MC4100 hns205::Tn10 Tetr10
Plasmids
    pPROBE-gfp[LVA]Promoterless gfp with LVA tag to decrease GFP half-life, Knr37
    pYELpPROBE-gfp[LVA] containing rovAYent promoter fragment from nt −614 to 170This study
    pYESpPROBE-gfp[LVA] containing rovAYent promoter fragment from nt −445 to 170This study
    pYPLpPROBE-gfp[LVA] containing rovAYpstb/Ypestis promoter fragment from nt −622 to 170This study
    pYPSpPROBE-gfp[LVA] containing rovAYpstb/Ypestis promoter fragment from nt −443 to 170This study
    pWKS30::StrSpecpWKS30 with a Str/Spec cassette in the HindIII site of the polylinker, Ampr Strr Specr10
    pHNSpWKS30::Str/Spec::hns with native promoter region10
    pLEUOpCR2.1::leuO with native promoter regionThis study