TABLE 3.

Summary of cis and trans mutations contributing to the smooth phenotypes of selected SARC isolates

Subspecies (group)IsolateComplementationaReversionbDefect(s) identified
Sarc1 mlrA trans regulatory changes
Sarc2 mlrA trans regulatory changes
S. enterica subsp. salamae (II)Sarc4 mlrA + rpoS PagfD cis mutation (OmpR binding site)Reduced RpoS activity, trans regulatory changes
Sarc35NonePagfD and PagfB cis mutations
Sarc37NonePagfD and PagfB cis mutations
S. enterica subsp. houtenae (IV)Sarc8 mlrA PagfD cis mutation (OmpR binding site) trans regulatory changes
Sarc16 rpoS PagfD cis mutation (−35 region)Reduced PrpoS activity, truncated RpoS
S. bongori (V)Sarc11None trans regulatory changes upstream of agfD trans regulatory changes
Sarc12NoneReduced RpoS activity, trans regulatory changes
S. enterica subsp. indica (VI)Sarc14 mlrA trans regulatory changes
  • a Isolates formed colonies of the rdar morphotype on T or LB agar at 28°C when transformed with genes on multicopy plasmid pBR322 or pACYC184.

  • b Pellicle-forming revertants were identified when SARC isolates were grown in 1% tryptone or LB standing culture at RT for 14 to 21 days.