TABLE 1.

Bacteria and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    E. coli Top10F mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupGInvitrogen
    E. coli S17-1thi proA hsdR17 hsdM+ recA RP4-tra function47
    R. eutropha H16Wild typeDSM 428a
    R. eutropha ΔphaP1phaP1 precise deletion gene replacement strain (Re1052), derived from R. eutropha H1658
Plasmids
    pEGFP-N3Kmr Neor egfp, pMB1 oriBD Biosciences
    pBBR1MCS-2Broad-host-range, Kmr lacPOZ mobRP419
    pJAM2::phaP1-egfppJAM2 harboring phaP1-egfp fusion12
    pBBR1MCS-2::phaP1ReupBBR1MCS-2 harboring PCR product comprising phaP1This study
    pBBR1MCS-2::egfppBBR1MCS-2 harboring PCR product comprising egfpThis study
    pBBR1MCS-2::phaP1Reu-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu-egfpThis study
    pBBR1MCS-2::egfp-phaP1ReupBBR1MCS-2 harboring PCR product comprising egfp-phaP1ReuThis study
    pBBR1MCS-2::phaP1Reu[52>C]-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu[52>C]-egfpThis study
    pBBR1MCS-2::phaP1Reu[113>C]-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu[113>C]-egfpThis study
    pBBR1MCS-2::phaP1Reu[ΔM]-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu[ΔM]-egfpThis study
    pBBR1MCS-2::phaP1Reu[52-112]-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu[52-112]-egfpThis study
    pBBR1MCS-2::phaP1Reu[1-85]-egfppBBR1MCS-2 harboring PCR product comprising phaP1Reu[1-85]-egfpThis study
    pBBR1MCS-2::egfp-phaP1Reu[N>85]pBBR1MCS-2 harboring PCR product comprising egfp-phaP1Reu[N>85]This study
    pBBR1MCS-2::egfp-phaP1Reu[N>112]pBBR1MCS-2 harboring PCR product comprising egfp-phaP1Reu[N>112]This study
    pBBR1MCS-2::egfp-phaP1Reu[ΔM]pBBR1MCS-2 harboring PCR product comprising egfp-phaP1Reu[ΔM]This study
    pBBR1MCS-2::egfp-phaP1Reu[52-112]pBBR1MCS-2 harboring PCR product comprising egfp-phaP1Reu[52-112]This study
  • a DSM, Deutsche Sammlung für Mikroorganismen und Zellkulturen.