TABLE 2.

Oligonucleotides used for PCR amplification and other purposes in this study

OligonucleotideSequenceLocation and orientation
PhaP1_N_XhoI5′-AAA CTC GAG AAG GAG GGA TCC ATG ATC CTC ACC CCG G-3′5′ region of phaP1Reu
EGFP_C_HindIII5′-AAA AAG CTT TTA CTT GTA CAG CTC GTC CAT GCC-3′3′ region of egfp
EGFP_C_EcoRI5′-G GCC GAA TTC CTT GTA CAG CTC GTC CAT GCC G-3′5′ region of egfp
EGFP_N_MCS(SalI)5′-T TCT GCA GTC GAC GGT ACC GC-3′3′ region of egfp
PhaP(Re)_C_HindIII5′-GC AGA AGC TTA TCA GGC AGC CGT CGT CTT C-3′3′ region of phaP1Reu
PhaP(Re)_N_EcoRI5′-G AGA GAA TTC ATG ATC CTC ACC CCG GAA CAA G-3′5′ region of phaP1Reu
PhaP-52C5′-A GAA CTC GAG GAG AAG GCC ATG AAG GCG CTG TCG GCC AAG-3′5′ region of truncated phaP1Reu
PhaP-N85-rev5′-GA AAA GCT TTC TTA CAG GTG GCG GGT GTA GGC-3′3′ region of truncated phaP1Reu
PhaP-113C5′-C GAA CTC GAG AAG AAC GTG CAA ATG CTG GTC GAG AAC CTC GCC-3′5′ region of truncated phaP1Reu
PhaP-N112-rev5′-AC CAA AGC TTA CAC GTT CTT CGA GCC TTC GG-3′3′ region of truncated phaP1Reu
P-EGFP-EcoRI-N5′-G AGG GAA TTC ATG GTG AGC AAG GGC GAG GAG C-3′5′ region of egfp
PhaP(112)_EcoRI_C5′-A AAA GAA TTC CAC GTT CTT CGA GCC TTC GG-3′3′ region of egfp
PhaP(52C)_EcoRI_N5′-A AAA GAA TTC AAG GCG CTG TCG GCC AAG-3′3′ region of truncated phaP1Reu
PhaP1-85_C_EcoRI5′-C CGA GAA TTC GCC GCC GCC GTG GCG GGT GTA GGC CAG G-3′3′ region of truncated phaP1Reu