TABLE 2.

Biofilm and primary adherence assays

StrainMedian (interquartile range)
OD570 for biofilm assayaPrimary adherenceb at:
30 min2 h
OG1RF1.257 (1.184-1.313)267 (238-316)649 (614-722)
TX5475 (ΔebpA)0.817 (0.766-0.882)97 (89-123)203 (168-229)
TX5515 (ΔebpR)0.667 (0.644-0.701)151 (114-231)314 (230-385)
TX5584 (ΔebpR)/pMSP35350.814 (0.750-0.891)166 (81-202)293 (260-429)
TX5582 (ΔebpR + ebpR)1.534 (1.487-1.674)221 (181-290)692 (527-750)
  • a The optical density at 570 nm (OD570) readings for the biofilm assays are from three independent experiments (total of 24 wells).

  • b Primary adherence was assessed after incubation for 30 min and 2 h. Bacteria in four fields from two independent plates from at least two independent experiments were subjected to light microscopy and counted.