TABLE 2.

Effect of virB1 deletion on virulence of A. tumefaciens on K. diagremontiana and complementation with virB1 and virB1 mutants

Strain (VirB1 variant)Tumor frequencyaMean relative tumor sizebRelative tumor index (%)c
A3480.9 (40)490
A348ΔB10.16 (40)0.51.8
A348ΔB1 (VirB1)1.0 (16)4100
A348ΔB1 (VirB1-LT)0.56 (16)228
A348ΔB1 (VirB1*)0.56 (16)228
A348ΔB1 (VirB1ΔSP)0.25 (16)16.25
A348ΔB1 [VirB1(AA/PR)]0.56 (16)228
A348ΔB1 [VirB1(E/Q)]0.43 (16)222
  • a Fraction of inoculations that incited tumors (number of inoculations).

  • b Size of tumors relative to that of tumors incited by A348 (wild type) estimated by visual inspection on a scale of 0 to 4.

  • c Calculated as: [(tumor frequency × relative tumor size)/4] × 100.