TABLE 3.

Yeast two-hybrid interactions between VirB1 constructs and VirB2 and VirB5 constructs

DNA-binding domain plasmidMean Miller units (SD) for activation domain plasmida:
pC-ACT.2.B2 (aa 1-121)pC-ACT.2.B2m (aa 48-121)pC-ACT.2.B2 (aa 95-121)pC-ACT.2.B5 (aa 24-220)pC-ACT.2
pCD.1.B16 (0.5)6 (0.8)8 (0.4)22 (0.9)0.02 (0.03)
pAS2.B1ΔSP29 (1.3)292 (3)9 (0.3)7 (0.2)ND
pCD.1.B1*+4480 (4.0)124 (9)3 (0.2)155 (9)−0.01 (0.02)
pCD.1.VirB1*11 (1.2)44 (2)3 (0.2)132 (3)ND
  • a n = 3. ND, no data because of poor growth.