TABLE 1.

Relevant bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coli
    CFT073Wild-type pyelonephritis isolate (O6:K2:H1) 45
    CFT073 fim L-ONCFT073 fim IE phase locked ON; constitutive type 1 fimbrial expression 27
    CFT073 fim L-ON Nalr Nalr derivative of CFT073 fim L-ONThis study
    CFT073 Δlrp CFT073 Δlrp::kan; replacement of nucleotides 41 to 428 of lrp in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 ΔlrhA CFT073 ΔlrhA::kan; replacement of nucleotides 41 to 889 of lrhA in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 ΔmutS CFT073 ΔmutS::kan; replacement of nucleotides 99 to 2332 of mutS in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 ΔydiV CFT073 ΔydiV::kan; replacement of nucleotides 91 to 552 of ydiV in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 ΔslyA CFT073 ΔslyA::kan; replacement of nucleotides 46 to 345 of slyA in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 ΔpapX CFT073 ΔpapX::kan; replacement of nucleotides 1 to 550 of papX in CFT073 with the kanamycin resistance gene of pKD4This study
    CFT073 fim L-ON Δlrp CFT073 fim L-ON Δlrp::kan; replacement of lrp (as in CFT073 Δlrp) in CFT073 fim L-ONThis study
    CFT073 fim L-ON ΔlrhA CFT073 fim L-ON ΔlrhA::kan; replacement of lrhA (as in CFT073 ΔlrhA) in CFT073 fim L-ONThis study
    CFT073 fim L-ON ΔmutS CFT073 fim L-ON ΔmutS::kan; replacement of mutS (as in CFT073 ΔmutS) in CFT073 fim L-ONThis study
    CFT073 fim L-ON ΔydiV CFT073 fim L-ON ΔydiV::kan; replacement of ydiV (as in CFT073 ΔydiV) in CFT073 fim L-ONThis study
    CFT073 fim L-ON ΔslyA CFT073 fim L-ON ΔslyA::kan; replacement of slyA (as in CFT073 ΔslyA) in CFT073 fim L-ONThis study
    CFT073 fim L-ON ΔpapX CFT073 fim L-ON ΔpapX::kan; replacement of papX, as in CFT073 ΔpapX, in CFT073 fim L-ONThis study
Plasmids
    pUT/mini-Tn5Km2Mini-Tn5 Km2 transposon in pUT suicide vector (Kanr Ampr) 18
    pKD4Lambda red recombinase system template plasmid (Kanr Ampr) 17
    pGEN-MCSDerivative of pGEN222 with multiple cloning site replacing gfpuv (Ampr) 33
    pGEN-lrp lrp (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr)This study
    pGEN-lrhA lrhA (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr)This study
    pGEN-mutS mutS (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr)This study
    pGEN-ydiV ydiV (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr)This study
    pGEN-slyA slyA (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr)This study
    pLX3607pQE60 cloning vector with lacIq cloned into XhoI site (Ampr) 37
    pDRM001 papX of CFT073 cloned into NcoI-HindIII sites of pLX3607 (Ampr) 37