TABLE 1.

MukB content of whole-cell extracts and isolated nucleoids

StrainaMukB content (pmol/mg) in the indicated fractionb
Cell extractNucleoidc
DH5α (pBAD)7.7 ± 1.728 ± 12 (>90)
DH5α (pBEF++)440320 (85)
DH5α (pEF++)10 ± 52.4 ± 0.8 (30 ± 20)
MG1655 (pEF)99 (93)
MG1655 (pBAD)12 ± 142 ± 10 (86 ± 2)
OU102 (pBAD)8 ± 54.0 ± 0.6 (57 ± 19)
MG1655 (pSmtA++)58 (96)
MG1655 (pTopA++)611 (71)
MG1655 (pB337++)813 (71)
  • a ++, plasmid designed to overproduce the indicated protein or fragment was induced with arabinose.

  • b The data are normalized to the total protein in a given fraction. When standard deviations are shown, the data are the average of two independent experiments.

  • c The percentage of the nuceloid-associated MukB that was released by DNase I treatment is given in parentheses.