TABLE 2.

Plasmids used in this study

PlasmidRelevant propertiesaSource or reference
pECM9Source of PaphA-gfp amplicon; Apr23
pECM20mobRP4, oriR6K integrates into X. nematophila chromosome to express gfp; Cmr23
pEVS107oriR6K, mobRP4, mobilizable suicide miniTn7-Erm delivery vector; Ermr Kmr34
pUX-BF13Mobilizable Tn7 transposition helper plasmid that expresses Tn7 transposase in trans; Apr2
pTn7/SR1pEVS107 parent, delivers miniTn7-Erm/nil7
pTn7/SR1/AM1ZpTn7/SR1 parent with nilA21 ATG to TAG point mutationThis study
pTn7/SR1/BM1ZpTn7/SR1 parent with nilB20 ATG to TAG point mutationThis study
pTn7/SR1/CM1ZpTn7/SR1 parent with nilC19 ATG to TAG point mutation7
pTn7/SR1/TnM1ZpTn7/SR1 parent with tn2 ATG to TAG point mutationThis study
pTn7/rrspEVS107 parent, integrates into X. poinarii rrsThis study
pTn7/SR1/rrspTn7/SR1 parent, integrates into X. poinarii rrsThis study
pTn7/SR1/AM1Z/rrspTn7/SR1/AM1Z parent, integrates into X. poinarii rrsThis study
pTn7/SR1/BM1Z/rrspTn7/SR1/BM1Z parent, integrates into X. poinarii rrsThis study
pTn7/SR1/CM1Z/rrspTn7/SR1/CM1Z parent, integrates into X. poinarii rrsThis study
pTn7/SR1/TnM1Z/rrspTn7/SR1/TnM1Z parent, integrates into X. poinarii rrsThis study
pTn7/rrs/GFPpTn7/rrs parent, integrates into Xenorhabdus rrs, constitutively expresses GFPThis study
pTn7/SR1/rrs/GFPpTn7/SR1/rrs parent, integrates into Xenorhabdus rrs, constitutively expresses GFPThis study
  • a Cm, chloramphenicol.