TABLE 1.

Colicin activities of various ColE9 mutants containing unique EK sites

ColicinEK sitea% lux inductionb
YZ23/Im96153.5
YZ49/Im98253.2
YZ52/Im928775.5
YZ39/Im94416.1
YZ25/Im947841.3
Free YZ2547841.3
  • a The position of the engineered EK site (i.e., the residue number) is indicated.

  • b lux induction is a measure of DNA damage and hence colicin activity and is expressed for each colicin construct relative to the ColE9 BH29/Im9, which is regarded as having 100% activity.