TABLE 1.

Streptococcal strains used in this work

SpeciesStrain (serotype)PhenotypeaAllelebAccession no.Description/reference(s)
S. pneumoniaeR6 (NTc)+1AE00848717
D39 (2)+1CP00041022
R6x (NT)+1AF10653940
TIGR4 (4)+1AE00567232
G54 (19F)+2AL449932; AM9012977 and this work
670 (6B)+3http://www.tigr.org
Rx1 (NT)+1AM90129624 and this work
JY2190 (NT)4AM90129839 and this work
JY2190 OptAs JY2190 but optochin resistant (5 μg ml−1); this work
Spain23F-1 (23F)+5http://www.Sanger.ac.uk/Projects/S_pneumoniae
INV104B (1)+6http://www.Sanger.ac.uk/Projects/S_pneumoniae
INV200 (14)+7http://www.Sanger.ac.uk/Projects/S_pneumoniae
OXC141 (3)+8http://www.Sanger.ac.uk/Projects/S_pneumoniae
SP9-BS68 (9V)+1NZ_ABAB0100001930
SP14-BS69 (14)+9NZ_ABAD0100000530
SP11-BS70 (11)+10NZ_ABAC0100001430
SP3-BS71 (3)+8NZ_AAZZ0100000730
SP23-BS72 (23F)+11NZ_ABAG0100000230
SP6-BS73 (6A)+12NZ_ABAA0100000730
SP18-BS74 (18C)+3NZ_ABAE0100000730
SP19-BS75 (19F)+5NZ_ABAF0100000730
R6Chi (NT)135
R6Chi CmAs R6Chi but chloramphenicol resistant (5 μg ml−1); this work
P023 (NT)14AM901299Cho-ind R6 transformant using SK598 DNA; this work
P072 (NT)14AM901310Cho-ind R6 transformant using P023 DNA; this work
P500 (NT)15AM901300Cho-ind R6 transformant using spr1150P072 (oligonucleotides atg and stop); this work
P501 (NT)16AM901301As P500 but isolated in an independent exp't; this work
P501 StrAs P501 but streptomycin resistant (150 μg ml−1); this work
P502 (NT)17AM901302Cho-ind R6 transformant using a PCR fragment of spr1150P072 (oligonucleotides a and b); this work
P511 (NT)18AM901303Cho-ind R6 transformant using a PCR fragment of spr1150P501 (oligonucleotides a and b); this work
P511 StrAs P511 but streptomycin resistant (150 μg ml−1); this work
P550 (NT)19AM901304Cho-ind R6 transformant using spr1150R6 (oligonucleotides atg and stop); this work
P551 (NT)20AM901305Cho-ind transformant using a PCR fragment of spr1150P550 (oligonucleotides dis, atg, and e); this work
P575 (NT)21AM901306Cho-ind R6 transformant using a PCR fragment of spr1150JY2190 (oligonucleotides atg and e); this work
P60022AM901307Cho-ind R6 transformant using a PCR fragment of spr1150SK598 (oligonucleotides a and b); this work
P70013Cho-ind R6 transformant using spr1150R6Chi (oligonucleotides atg and stop); this work
M222+NDA multiresistant strain; 10
S. pseudopneumoniaeCCUG 49455T+23AM9013082 and this work
S. mitisSK59824AM9013093 and this work
NCTC 12261T25http://www.tigr.org
  • a + and − indicate choline-dependent and Cho-ind strains, respectively.

  • b Only the last 1,462 nucleotides (out of 1,488 nucleotides) of the spr1150 gene were compared. ND, not determined.

  • c NT, nontypeable.