TABLE 1.

Bacteria and plasmids used

Strain or plasmidGenotype or descriptionSource or reference
Strains
    CF4941MG1655 galK2 zib563::Tn10 ΔrelA251M. Cashel
    CF4977CF4941 spoT202M. Cashel
    CF4943CF4941 spoT203M. Cashel
    MC4100F araD139 Δ(argF-lac)205λ rpsL150 flbB5301 relA1 deoC1 pstF25CGSCa
    SBG231AB259 spontaneous MccJ25r mutant (rpoC T931I)13
    AB1133F thr-1 ara-14 leuB6 lacY1 Δ(gpt-proA)62 supE44 galK2 λ rac hisG4 rfbD1 rpsL31 (Smr) kdgK51 xyl-5 mtl-1 argE3 thi-1CGSC
    RO98MC4100 ΔrelA251::kan ΔspoT207::catR. Hengge-Aronis
    SBS100MC4100 yojI::lacZS. B. Socías
Plasmids
    pCNB0209RPlac-His-relA8
    pALS13Ptac-relA′ (RelA 1-455); Apr41
    pPAV01pACYC184 carrying fhuAP. A. Vincent
    pFP348pACYC184 carrying mcjABCThis study
  • a CGSC, E. coli Genetic Stock Center.