TABLE 3.

Oligonucleotides used in this study

Oligonucleotide5′ to 3′ sequenceaPurpose
AttTn7ExtTGTTGGTTCACATCCTn7 insert amplification
ErmAnch1TACTTATGAGCAAGTATTGTCTn7 insert amplification
AMtoZfornewGACAAAATGACTCTAGATTTCTAATTATTATCCTAATCTGCMutagenesis
AMtoZrevnewGCAGATTAGGATAATAATTAGAAATCTAGAGTCATTTTGTCMutagenesis
BMtoZforTGGATTTGATTTTTTTCTAAGAGTTTTCATTGGTAATGMutagenesis
BMtoZrevTACCAATGAAAACTCTTAGAAAAAAATCAAATCCATCGMutagenesis
TnMtoZforTTTATGGATGTATTCGCTCTACCATTTAGTTTACGCCGCMutagenesis
TnMtoZrevGCGTAAACTAAATGGTAGAGCGAATACATCCATAAAAGCMutagenesis
pKmGFPEcoForGAATTCGTTGTGTCTCAAAATCTCTGgfp amplification
pKmGFPEcoRevGAATTCGGATATAGTTCCTCCTTTCAGCgfp amplification
  • a Underlined regions indicate ATG to TAG null mutations.