TABLE 1.

Bacterial strains and plasmid used

Strain or plasmidPropertiesDerivation or referencea
Strains
    CS093Wild-type S. enterica serovar TyphimuriumATCC 14028
    CS015CS093 phoP102::Tn10d-cam21
    CS022CS093 pho-24 (PhoP constitutive)21
    CS401CS093 phoN2 zxx-6251::Tn10d-cam rpsL (Strr)22
    CS491CS022 rpsLTransduction of rpsL from CS401
    TG241CS491 lpxO
    WLP97CS491 pagP
    TG273CS491 lpxO pagP
    TG275CS491 pagP lpxO pmrA::Tn10d-tetTransduction of pmrA from CS338 (= JSG421)
    CS338Also JSG421; pmrA::Tn10d-tet22
    HN1138CS093 tolC::kanTransposon mutagenesis
    HN1139CS015 tolC::kanTransduction of tolC from HN1138
    HN1140CS022 tolC::kanTransduction of tolC from HN1138
    HN1141CS491 tolC::kanTransduction of tolC from HN1138
    HN1142TG241 tolC::kanTransduction of tolC from HN1138
    HN1143WLP97 tolC::kanTransduction of tolC from HN1138
    HN1144TG273 tolC::kanTransduction of tolC from HN1138
    HN1145TG275 tolC::kanTransduction of tolC from HN1138
    HN1147CS491 phoP102::Tn10d-cam tolC::kanTransduction of phoP from CS015 into HN1141
    HN1148CS022 pmrA::Tn10d-tet tolC::kan rpsLSuccessive transductions of pmrA and rpsL into HN1140
    D21E. coli K-12 unaltered for LPSE. coli Genetics Stock Center
    D21f2“Deep rough” mutant lacking the R core entirelyE. coli Genetics Stock Center
Plasmid
    pKAS32Ampr Strs (rpsL+); low-copy-number allelic exchange vector39
  • a —, in-frame deletions were introduced by using pKAS32.