TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristic(s)aSource or reference
Strains
    S. thermophilus
        LMD-9Wild typeATCCb
        LF101LMD-9 ΔblpR St This study
        LF102LMD-9 ΔblpH St This study
        LF103LMD-9 Δ(blpR St-blpH St)This study
        LF104LMD-9 Δ(blpD St-blpX St)This study
        LF105LMD-9 Δ(blpD St-blpF St)This study
        LF106LMD-9 Δ(blpG St-blpX St)This study
        LF107LMD-9 ΔblpB St This study
        LF108LMD-9 Δ(blpA St-blpB St)This study
        LMG18311Wild type 8
        CNRZ1066Wild type 8
    E. coli
        TG1 supE hsdΔ5 thi Δ(lac-proAB) F′[traD36 proAB+ lacIq lacZΔM15] 42
        EC1000Kmr RepA+; MC1000 containing a copy of the repA gene of pWV01 in its chromosome 33
Plasmids
    pMG36eEmr; E. coli-S. thermophilus shuttle vector; contains the P32 promoter 44
    pGIBG001Emr; pMG36e where a NcoI restriction site has been introduced in order to allow the cloning of genes in translational fusion with the P32 promoterThis study
    pGILF001Emr; pGIBG001 with a 0.16-kb insert containing the StblpC ORF of S. thermophilus LMG18311 in translational fusion the P32 promoterThis study
    pGILF002Emr; pGIBG001 with a 0.16-kb insert containing the StblpC ORF of S. thermophilus LMD-9 in translational fusion the P32 promoterThis study
    pGhost9Emr Ts 35
  • a Emr and Kmr indicate resistance to erythromycin and kanamycin, respectively, and Ts indicates a temperature-sensitive replicative plasmid.

  • b American Type Culture Collection, Rockville, MD.