TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmidaDerivation, genotype, or phenotypeH antigen expressedFlagellin genotypeReference or source
Strains
    G1436Bi7327-41, H3 type strainH3 fliC 16off flkA 3 on 30
    G13934370-53, H35 type strainH35 flkA 35 on This study
    G13945017-53, H36 type strainH36 flkA 36 on 28
    G13971755-58, H47 type strainH47 fliC 21 off flkA 47 on 28
    G1392E480-68, H53 type strainH53 flkA 53 on 28
    H1171Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1172Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1173Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1174Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1175Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1176Phase variant of G1436H16 fliC 16 on flkA 3 none This study
    H1201Phase variant of G1397H47 fliC 21 on flkA 47 none This study
    H1202Phase variant of G1397H47 fliC 21 on flkA 47 none This study
    G1438b EJ34 11
    G1370DH5α
    G1067H3 non-type strainH3 fliC 16 off flkA 3 on
    G1145H47 non-type strainH47 fliC 21 off flkA 47 on
    H1692 orf486-deficient mutant of G1436H3 fliC 16 off flkA 3 on This study
    H1693H1692 carrying pLW1330H3 fliC 16 off flkA 3 on This study
    H1694G1436 carrying pLW1330H3 fliC 16 off flkA 3 on This study
Plasmids
    pUC19Cloning vector; Apr Takara
    pLW1249pUC19 with a 8.5-kb insert containing the flagellin gene for H35This study
    pLW1041pUC19 with a 4.1-kb insert containing the flagellin gene for H36This study
    pLW1248pUC19 with a 3.1-kb insert containing the flagellin gene for H47This study
    pKD4Contains a kanamycin resistance gene (kan); Kmr Apr 8
    pKD20RED recombinase expression plasmid; Apr 8
    pUC18Cloning vector; Apr Takara
    pLW1330pUC18 containing orf486 This study
  • a E. coli H antigen type strains were obtained from the Institute of Medical and Veterinary Science, Adelaide, Australia, and the Mechnikov Research Institute for Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia. Other E. coli standard H type strains used have been described previously (12, 46).

  • b A derivative of E. coli K-12 which is nonflagellated and nonmotile because the expression of its single flagellin gene, fliC, is blocked by a mutation.