TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsReference
Strains
    AM604MC4100 ara+43
    AM661AM604 ΔlptD::kan/araBp-lptD20
    AM679AM604 ΔlptE::kan/araBp-lptEThis study
    AM689AM604 ΔlptE::kan Δ(λatt-lom)::bla araBp-lptE43
    BB-3BW25113 Φ(kan araC araBp-lptC)136
    BB-4BW25113 Φ(kan araC araBp-lptA)136
    BW25113Δ(araD-araB)567 ΔlacZ4787(::rrnB-4) lacIq λ rpoS396(Am) Δ(rhaD-rhaB) rrnB-4 hsdR51411
    DH5αΔ(argF-lac169) φ80dlacZ58(M15) glnV44(AS) λ rfbD1 gyrA96 recA1 endA1 spoT1 thi-1 hsdR1715
    DY378W3110 λcI857 Δ(cro-bioA)44
    FL750AM661 ΔwaaL::camThis study
    FL752AM679 ΔwaaL::camThis study
    FL905AM604 Φ(kan araC araBp-lptC)1This study
    FL907AM604 Φ(kan araC araBp-lptA)1This study
    FL909MC4100-BB3 ΔwaaL::camThis study
    FL911MC4100-BB4 ΔwaaL::camThis study
    MC4100F araD139 Δ(argF-lac)U169 rpsL150 relA1 flb5301 deoC1 ptsF25 thi7
    MG1655LAM rph-11
Plasmids
    pBAD18Arabinose-inducible expression vector, Ampr14
    pBADlptEpBAD18 araBp-lptEThis study
    pGS100pGZ119EH derivative, contains TIR sequence downstream of ptac36
    pGS108pGS100 ptac-lptC-His6This study
    pKD3oriRγ; Ampr Camr; source of cat cassette11
    pKD4oriRγ; Ampr Kanr; source of kan cassette11