TABLE 1.

Plasmids used in this study

PlasmidDescriptionSource or reference
pCLD1Cmr derivative of pEVS107; Kanr Cmr This study
pCLD6pCLD1 + 3.2-kb ApaI/SpeI fragment from pEAH41 containing the psypA-lacZ fusion; Kanr Cmr This study
pCLD19Delivery vector carrying sequences flanking sypE This study
pCLD46pVSV105 carrying the rscS1 allele; Cmr This study
pCLD48pVSV105 carrying wild-type sypE; Cmr This study
pCR2.1-TOPOCommercial cloning vector; Apr Kanr Invitrogen, Carlsbad, CA
pEAH41pTMO82 carrying psypA upstream of lacZ; Apr Kanr This study
pEAH73pKV69 carrying wild-type sypG; Cmr Tcr This study
pES420Mobilizable suicide vector, Emr 21a
pEVS107Mini-Tn7 delivery plasmid; mob; Kanr Emr 18
pKG11pKV69 carrying rscS1 allele; Cmr Tcr 42
pKV69Mobilizable vector; Cmr Tcr 37
pKV276pEAH73 with D53E mutation in sypG; Cmr Tcr This study
pTMO82pCR2.1-TOPO carrying promoterless lacZ; Apr Kanr This study
pVSV105Mobilizable vector; Cmr 6