TABLE 1.

Plasmids and strains used in this study

Strain or plasmidGenotype, characteristics, or constructionSource or reference
E. coli strains
    TAM1mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupGActive Motif
    DH5αendA1 hsdR17(rK mK+) glnV44 thi-1 recA1 gyrA (Nalr) relA Δ(lacIZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15]48
    TOP10 F′F′ [lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
V. fischeri strains
    ES114Wild type4
    KV2566ES114 att Tn7::PsypA::lacZ15a
Plasmids
    pJET1Commercial cloning vector, AmprFermentas
    pCR2.1-TOPOCommercial cloning vector, Ampr KanrInvitrogen
    pKV69Mobilizable vector, Cmr Tetr47
    pKG63pKV69 containing rscS1 under control of PlacZ, Cmr Tetr15a
    pKG92pKV69 containing rscS1(ΔPP-large), Cmr TetrThis study
    pKG94pKV69 containing rscS1(ΔPP-small), Cmr TetrThis study
    pKG99pKV69 containing rscS1(H323A), Cmr TetrThis study
    pKG100pKV69 containing rscS1(F353A), Cmr TetrThis study
    pKG102pKV69 containing rscS1(N345A), Cmr TetrThis study
    pKG107pKV69 containing rscS1(H412Q), Cmr TetrThis study
    pKG108pKV69 containing rscS1(H867Q), Cmr TetrThis study
    pKG115pKV69 containing rscS1(ΔN-term), Cmr TetrThis study
    pKG116pKV69 containing rscS1(L330R), Cmr TetrThis study
    pKG123pKV69 containing rscS1(S9T), Cmr TetrThis study
    pKG124pKV69 containing rscS1(S9A), Cmr TetrThis study
    pKG125pKV69 containing rscS1(I13A), Cmr TetrThis study
    pKG127pKV69 containing rscS1(D709A), Cmr TetrThis study