TABLE 1.

Purification of hydroxyquinol 1,2-dioxygenase from Arthrobacter sp. strain JS443

Purification stepTotal protein (mg)Total activity (μmol min−1)Sp act (μmol min−1 mg protein−1)Yield (%)
Crude lysate620120.019
Ammonium sulfate610180.029
Dialysis4602000.43100
Q-Sepharose Fast Flow6.8951448
Sephacryl 1000.95303215
Phenyl Superose0.126.7583.3