TABLE 1.

Strains and plasmids used in this study

Strain or plasmidSerotypeOrigin, genotype, and/or phenotypeReference or source
Streptococcus pneumoniae strains
    R6United States4
    TIGR44Norway1
    6289VSpain43
    Hu1519AHungary39
    496a19FSpain, 198743
    456a23FSpain, 198443
    632a23FSpain, 198743
    637a23FSpain, 198743
    638a23FSpain, 198743
    653a23FSpain, 198743
    673a23FSpain, 198743
    674a23FSpain, 198743
    677a23FSpain, 198743
    SA16a23FSouth Africa43
    SA17a23FSouth Africa43
    F4a23FFranceCollection of Kaiserslautern University
    F14a23FFranceCollection of Kaiserslautern University
    230623FFinland, 198543
    2306spiB::pJDB223FspiB::pJDB2 mutant of 2306This study
    2306spiR::pJDR123FspiR2::pJDR1 mutant of 2306This study
    2306pncO::pJDO323FpncO::pJDO3 mutant of 2306This study
    2306pncP::pJDP423FpncP::pJDP4 mutant of 2306This study
    2306ΔpncR-K23FpncR-K deletion mutant of 2306This study
    TIGR4ΔpncA-N4pncA-N deletion mutant of TIGR4This study
Other bacteria
    Streptococcus salivarius 674Collection of Kaiserslautern University
    Streptococcus oralis 510Collection of Kaiserslautern University
    Streptococcus pyogenes spy 15Collection of Kaiserslautern University
    Streptococcus sanguis DSM 2056728
    Streptococcus mitis NTCC1071226
    Staphylococcus aureus 113Collection of Kaiserslautern University
    Lactococcus lactis MG136351
    Micrococcus luteus DSM278642
    Enterococcus faecalis ATCC 2921245
    Listeria ivanovii21
    Listeria welshimeri21
    Bacillus subtilis DB 403Collection of Kaiserslautern University
    Pseudomonas putida DSM5090653
    Escherichia coli JM10954
    Escherichia coli invα F′Promega
Plasmids
    pJDC9Eryr; nonreplicative in S. pneumoniae8
    pJDR1Eryr; pJDC9 derivative carrying an internal DNA fragment of spiR2This study
    pJDB2Eryr; pJDC9 derivative carrying an internal DNA fragment of spiBThis study
    pJDO3Eryr; pJDC9 derivative carrying an internal DNA fragment of pncOThis study
    pJDP4Eryr; pJDC9 derivative carrying an internal DNA fragment of pncPThis study
    pGEM-T EasyAmprPromega
    pGEM-ABAmpr; pGEM-T Easy derivative carrying a fragment of spiA and pncOThis study
    pGEM-ASBAmpr Spcr pGEM-T Easy derivative carrying a fragment of spiA and pncO and the aad9 geneThis study
  • a Member of the multiresistant Spain23F-1 clone.