TABLE 3.

Transformation rates for E. coli lysate containing NpdA2

SubstrateDegradation rate (nmol/min/mg lysate protein)ProductAccumulation rate (nmol/min/mg lysate protein)
4-Chlorocatechol14.0 ± 0.7Hydroxyquinol7.8 ± 0.9
Differencea6 ± 1
ChlorohydroquinoneNDbHydroxyquinolND
m-ChlorophenolNDChlorohydroquinoneND
p-Chlorophenol18 ± 2Hydroquinone4 ± 1
Hydroxyquinol15 ± 1
4-Chlororesorcinol18 ± 2Hydroxyquinol16.6 ± 0.9
m-CresolNDMethylhydroquinoneND
p-CresolNDHMCHND
2,4-Dichlorophenol14.5 ± 0.9Chlorohydroquinone3.1 ± 0.2
Hydroxyquinol7 ± 2
Difference4.1 ± 0.9
2,4-DinitrophenolNDNitrohydroquinoneND
4-MethylcatecholND5-Methylhydroxy-quinolND
4-Nitrocatechol15.7 ± 0.9Hydroxyquinol15.8 ± 0.3
m-Nitrophenol10 ± 1Nitrohydroquinone9 ± 1
PNP31 ± 2Hydroquinone7 ± 2
Hydroxyquinol25 ± 0.9
PhenolNDHydroquinoneND
2,4,5-TrichlorophenolND2,5-Dichloro-hydroquinoneND
5-Chlorohydroxy-quinolND
2,4,6-TrichlorophenolND2,6-Dichlorohydro-quinoneND
6-Chlorohydroxy-quinolND
UnidentifiedND
  • a Difference between rate of substrate disappearance and sum of the rates of product accumulation. Some or all of the difference is due to accumulation of a product which has been identified as 5-chlorohydroxyquinol based on MS characteristics, but for which an authentic standard with a known concentration is unavailable.

  • b ND, not determined.