TABLE 2.

Plasmids used during this study

PlasmidRelevant characteristicsSource or referencea
pKD46bla PBAD gam beto exo pSC101 oriTS10
pCP20bla cat cI857 λPRflp pSC101 oriTS8
pKD3bla FRT cm FRT oriR6K10
pKD4bla FRT kan FRT oriR6K10
pPROBE GFP [tagless]kan GFP [tagless] ori p15a46
pSS001 (PflgA-GFP)kan PflgA-GFP [tagless] ori p15a
pSS002 (PflgB-GFP)kan PflgB-GFP [tagless] ori p15a
pSS003 (PflhB-GFP)kan PflhB-GFP [tagless] ori p15a
pSS004 (PfliE-GFP)kan PfliE-GFP [tagless] ori p15a
pSS005 (PfliD-GFP)kan PfliD-GFP [tagless] ori p15a
pSS006 (PflgK-GFP)kan PflgK-GFP [tagless] ori p15a
pSS007 (PfliC-GFP)kan PfliC-GFP [tagless] ori p15a
pSS008 (PflhD-GFP)kan PflhD-GFP [tagless] ori p15a
pSS009 (pPROBE luxCDABE)kan luxCDABE ori p15a
pSS010 (PflgA-luxCDABE)kan PflgA-luxCDABE ori p15a
pSS011 (PfliC-luxCDABE)kan PfliC-luxCDABE ori p15a
pPROTet.Ecm PLtetO-1 ori ColE1Stratagene
pSS012 (pPROTet.E tetR)cm PLtetO-1 tetR ori ColE1
pSS013 (pFliZ)cm PLtetO-1 fliZ ori ColE1
pSS014 (pFliZ-TetR)cm PLtetO-1 fliZ tetR ori ColE1
pSS015 (pFliZ-native)cm PfliA fliZ ori ColE1
pSS016 (pFliA-TetR)cm PLtetO-1 fliA tetR ori ColE1
pSS017 (pFlhDC-TetR)cm PLtetO-1 flhDC tetR ori ColE1
pSUB11bla 3×FLAG FRT kan FRT oriR6K54
pSS018 (PflhDC flhDC-lacZ)kan PflhDC flhDC-lacZ ori p15a
pSS019 (PLtetO-1 flhC-lacZ)cm PLtetO-1 flhC-lacZ ori ColE1
pSS019 (PLtetO-1 flhC-lacZ)cm PLtetO-1 flhC-lacZ ori ColE1
pSS020 (PLtetO-1 flhDC-lacZ)cm PLtetO-1 flhDC-lacZ ori ColE1
  • a Plasmids are from this study unless specified otherwise.