TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant feature(s)Source or reference
Strains
    A. tumefaciens strains
        C58Nopaline type strain; pTiC58, pAtC5842
        PMM3ΔcheA C58 derivativeThis study
        PMM4ΔflgE C58 derivativeThis study
        PMM5ΔmotA C58 derivativeThis study
        cms-1 mutantSpontaneous suppressor of PMM3This study
    E. coli strains
        SM10/λpirλpir Tra+; cloning strain29
        SY327/λpirλpir; cloning strain30
        TOP10 F′Cloning strainInvitrogen
Plasmids
    pGEM-T EasyPCR cloning vector; AprPromega
    pBBR1MCS-2Broad-host-range Plac expression vector; Kmr22
    pKNG101R6K ori; sacB (Sucs) Smr18
    pJZ383Ptac::gfpmut3 Spr; pVS repliconJ. Zhu (9)
    pPM104pKNG101 carrying cheA SOE deletion fragmentThis study
    pPM107pKNG101 carrying flgE SOE deletion fragmentThis study
    pPM108pKNG101 carrying motA SOE deletion fragmentThis study
    pPM109pBBR1MCS-2 Plac::cheAThis study
    pPM110pBBR1MCS-2 Plac::flgEThis study
    pPM111pBBR1MCS-2 Plac::motAThis study