TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference
Strains
    E. coli
        W3110IN(rrnD-rrnE)1 rph-112
        DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK mK+) gal phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
        CLM24W3110 lacking WaaL ligase12
        SΦ874lacZ trp Δ(sbcB-rfb) upp rel rpsL27
        SCM3SΦ874 ΔwaaLValvano et al., unpublished data
        SCM7SΦ874 Δwec3
    P. aeruginosa
        1244Wild-type, serotype O76
        1244 pilO1244 lacking PilO6
Plasmids
    pSPORT1Cloning vector, AmprInvitrogen
    pMLBADCloning and expression vector, arabinose inducible, Tpr22
    pEXT20Cloning and expression vector, IPTG inducible, Ampr10
    pEXT21Cloning and expression vector, IPTG inducible, Spr10
    pEXT22Cloning and expression vector, IPTG inducible, Kmr10
    pPAC46Encodes P. aeruginosa 1244 pilA-pilO operon, Ampr6
    pACYCpglCarries the C. jejuni protein glycosylation locus (pgl) cluster, Cmr45
    pACYCpglBmutCarries C. jejuni pgl containing mutations W458A and D459A in PglB, Cmr45
    pACYCpglKmutCarries C. jejuni pgl containing a Kmr cassette in pglK, Cmr and Kmr3
    pJHCV32Encodes the O7 antigen cluster from E. coli, Tetr26
    pJHCV32::Tn3HoHo1-134Encodes the O7 antigen cluster from E. coli carrying a transposon in wzy, Tetr Ampr26
    pJHCV32::Tn3HoHo1-136Encodes the O7 antigen cluster from E. coli carrying a transposon in wzz, Tetr Ampr26
    pCW27pglK in pMLBAD/Myc-His6, Tpr3
    pDOM2pilO subcloned in pSPORT1, AmprThis study
    pDOM3pilO subcloned in pMLBAD, TprThis study
    pAMF1pilA subcloned in pEXT20, AmprThis study
    pAMF3pilE cloned in pEXT20, AmprThis study
    pAMF4His10-tagged pglL cloned in pSPORT1, AmprThis study
    pAMF5His10-tagged pglL cloned in pEXT22, KmrThis study
    pAMF6pilE cloned in pEXT21, SprThis study
    pAMF14His6-tagged pilE cloned in pEXT21, SprThis study