TABLE 2.

Oligonucleotides utilized in this study

DesignationNucleotide sequence
Cys2R5′-GCAGGTGACGGCAGGTGC-3′
Erm1.5Rev5′-TGTTAGCCAAAGCTTCCAAGC-3′
HVPilS(R)5′-CGTTGTCGTTGCCGGCTTTGGT-3′
InterB65′-GCCTGAAGGTAATAGTGCTGGTGCGCGACGGC-3′
PilRBS5′-GGCTTTCCCCTTTCAATTAGGAG-3′
RecC25′-GATTTGTTCCGCAGCGCACAA-3′
RecC45′-GGCAACGTATTGATGATTCG-3′
RecB45′-CCCAAAGCTTGCACTGCACAAATGGCTGCGCGATCAAATC-3′
RecB55′-CTAGTCTAGAGTGCGGGCAGGAAGGTGTCGCCTTCGT-3′
RecB65′-CTAGTCTAGAGGTGAGGGTGTGCGTGGTCATA-3′
RecB95′-CCCTCGAGGCTCAAGATTTTTGGCGGGAACG-3′
RecB105′-GGTCTAGACCCGAAGTCTGCCAGTTTCTGCAATTC-3′
RecB125′-AAAAGCTTGGCTGTGCCTGCACGAAATTCTTGAAGA-3′
Tracy15′-CGATATGGTCTGCCAAGACGACGGCAATATCTGC-3′
Tracy25′-GCAGATATTGCCGTCGTCTTGGCAGACCATATCG-3′
GC recB15′-AAACCGCAACCCCGCCGCA-3′
GC recB25′-GCGTGGGACGCGCAAGATACC-3′
GC recB35′-CGGCGGCGTTGCCAGCGT-3′
GC recB45′-GGTGCCGCCGTCCCGAA-3′
probe 2455′-GCCTTTTTGAAGGGTATTCAT-3′