TABLE 1.

Bacterial strains utilized in this study

StrainDescriptionSource or reference
Escherichia coli
    DH5αF φlacZ Δ(lacZYA argF)U16 deoR recA1 endA1 hsdR17(rκ mκ+)phoA supE44 λthi-1 gyrA96 relA1Gibco BRL, Gaithersburg, MD
    GY5873Hfr argA::Tn9 lacMS286 Φ80dII lacBK1 recB2121
Neisseria gonorrhoeae
    MS11 rec+Wild typeJ. Swanson
    P9 rec+Wild typeJ. Saunders
    FA1090 rec+Wild typeF. Sparling
    MS11 recBrecB::ermCThis study
    MS11 recB opaE::recB+Wild type recB gene placed in opaE locusThis study
    MS11 recB supIntragenic recB suppressor mutantThis study
    FA1090 recBrecB::ermCThis study
    P9 recBrecB::kanThis study
    MS11 proΔProline residue in recB active site and ermC cassette 3′ of recB geneThis study
    MS11 inv900-bp segment of the pilE locus in an inverse orientationThis study
    MS11 recCrecC::ermC (or kan)This study
    FA1090 recCrecC::ermC (or kan)This study
    P9 recCrecC::ermC (or kan)This study
    MS11 Sma::XhoXhoI linker placed in the SmaI site in the Sma/Cla repeat downstream of pilE9
    FA1090 Sma::XhoXhoI linker placed in the SmaI site in the Sma/Cla repeat downstream of pilEThis study