TABLE 1.

Bacterial strains utilized in this study

StrainDescriptionSource or reference
Escherichia coli
    DH5αF φlacZ Δ(lac ZYA argF)U16 deo R rec A1 endA1 hsdR17(rκ mκ +)pho A supE44 λ thi-1 gyrA96 relA1 Gibco BRL, Gaithersburg, MD
    GY5873Hfr argA::Tn9 lacMS286 Φ80dII lacBK1 recB21 21
Neisseria gonorrhoeae
    MS11 rec + Wild typeJ. Swanson
    P9 rec + Wild typeJ. Saunders
    FA1090 rec + Wild typeF. Sparling
    MS11 recB recB::ermC This study
    MS11 recB opaE::recB + Wild type recB gene placed in opaE locusThis study
    MS11 recB sup Intragenic recB suppressor mutantThis study
    FA1090 recB recB::ermC This study
    P9 recB recB::kan This study
    MS11 pro ΔProline residue in recB active site and ermC cassette 3′ of recB geneThis study
    MS11 inv 900-bp segment of the pilE locus in an inverse orientationThis study
    MS11 recC recC::ermC (or kan)This study
    FA1090 recC recC::ermC (or kan)This study
    P9 recC recC::ermC (or kan)This study
    MS11 Sma::XhoXhoI linker placed in the SmaI site in the Sma/Cla repeat downstream of pilE 9
    FA1090 Sma::XhoXhoI linker placed in the SmaI site in the Sma/Cla repeat downstream of pilE This study