TABLE 1.

Bacterial strains used in this study

StrainDescriptionSource or reference
E. coli DH5αdeoR endA1 gyrA96 hsdR17(rK mK+) recA1 relA1 supE44 λ thi-1 Δ(lacZYA-argF)U16922
L. monocytogenes
    EGD-eWild type, derivative of EGDG. B. Mackaness
    EGD-e::hprK (lmo2483)Inactivation of HPrK by insertion of pLSV101 in lmo248333
    EGD-e::ptsH (lmo1002)Inactivation of HPr by insertion of pLSV101 in lmo100233
    EGD-eΔlmo1167 (ΔglpF2)In-frame deletion of lmo1167 (glycerol uptake facilitator)This study
    EGD-eΔlmo1539 (ΔglpF1)In-frame deletion of lmo1539 (glycerol uptake facilitator)This study
    EGD-eΔlmo1034 (ΔglpK2)In-frame deletion of lmo1034 (glycerol kinase)This study
    EGD-eΔlmo1538 (ΔglpK1)In-frame deletion of lmo1538 (glycerol kinase)This study
    EGD-eΔlmo1538-39 (ΔglpFK1)In-frame deletion of lmo1538 (glycerol kinase) and lmo1539 (glycerol uptake facilitator)This study
    EGD-eΔlmo1293 (ΔglpD)In-frame deletion of lmo1293 (glycerol-3-P dehydrogenase)This study
    EGD-eΔlmo1293 (ΔglpD)-CEGD-eΔlmo1293 (ΔglpD) complemented with glpDThis study
    EGD-eΔlmo1538 (ΔglpK1)-CEGD-eΔlmo1538 (ΔglpK1) complemented with glpK1This study