TABLE 4.

Genes differentially regulated in glucose compared to cellobiose at early log phase (phase A) and late log phase (phase B) in MMa

GeneFunctionFold induction
GC-AGC-B
lmo0018Beta-glucosidase0.2
lmo0096bSimilar to PTS; mannose-specific, factor IIAB15.810.3
lmo0097Similar to PTS; mannose-specific, factor IIC13.49.5
lmo0098bSimilar to PTS; mannose-specific, factor IID12.68.6
prfAcListeriolysin positive regulatory protein5.846.2
plcAb,cPhosphatidylinositol-specific phospholipase c9.248.6
hlyb,cListeriolysin O precursor10.446.1
mplcZinc metalloproteinase precursor4.547.3
actAcActin assembly-inducing protein precursor15.264.8
plcBb,cPhospholipase C12.6117
lmo0271Highly similar to phospho-beta-glucosidase0.3
inlAb,cInternalin A4.57.7
inlBb,cInternalin B3.25.9
lmo0560Similar to NADP-specific glutamate dehydrogenase2.1
uhpTb,cHighly similar to hexose phosphate transport protein61.8
lmo0914Similar to PTS, IIB component4.1
pheSPhenylalanyl-tRNA synthetase alpha subunit0.3
pheTPhenylalanyl-tRNA synthetase beta subunit0.4
tcsACD4+ T-cell-stimulating antigen; lipoprotein0.5
zurAMetal (zinc) transport protein(ABC transporter, ATP-binding protein)1.9
valSValyl-tRNA synthetase3.4
aroA3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase0.5
lmo1625Similar to putative transporters0.4
trpAHighly similar to tryptophan synthase (alpha subunit)2.90.3
trpBHighly similar to tryptophan synthase (beta subunit)4.70.3
trpFPhosphoribosyl anthranilate isomerase70.4
trpCHighly similar to indol-3-glycerol phosphate synthases7.80.4
trpDHighly similar to anthranilate phosphoribosyltransferase8.10.4
lmo1719Similar to PTS lichenan-specific enzyme IIA component0.4
lmo1734Similar to glutamate synthase (large subunit)2
inlCcInternalin C55.5
ilvBSimilar to acetolactate synthase (acetohydroxy acid synthase) (large subunit)2
ilvNSimilar to acetolactate synthase (acetohydroxy acid synthase) (small subunit)2
ilvCbSimilar to ketol acid reductoisomerase (acetohydroxy acid isomeroreductase)1.9
ilvASimilar to threonine dehydratase1.9
lmo2114Similar to ABC transporter (ATP-binding protein)7.2
lmo2115Similar to ABC transporter (permease)7
arpJSimilar to amino acid ABC transporter; permease protein0.5
lmo2390Similar to hypothetical thioredoxin reductase1.9
lmo2469Similar to amino acid transporter2.3
glyAHighly similar to glycine hydroxymethyltransferase0.4
lmo2580Similar to ABC transporter; ATP-binding protein2.3
lmo2650bSimilar to hypothetical PTS EIIB component3.4
lmo2651Similar to mannitol-specific PTS EIIA component2.7
lmo2684Similar to cellobiose phosphotransferase EIIC component0.10.3
lmo2685Similar to cellobiose phosphotransferase EIIA component0.10.2
cydDHighly similar to ABC transporter (ATP-binding protein) required for expression of cytochrome bd0.5
cydCHighly similar to ABC transporter required for expression of cytochrome bd0.5
serSSeryl-tRNA synthetase0.4
  • a Values indicate regulation, and no value indicates no regulation of the gene under the conditions mentioned. The genes discussed in this study are indicated in boldface type. The complete list of differentially regulated genes are available in Table S2 in the supplemental material. G, glucose; C, cellobiose; A, phase A; B, phase B.

  • b Gene regulated in the hprK mutant (33).

  • c Gene shown to be directly regulated by PrfA.