TABLE 1

Bacterial strains used in this study

StrainRelevant feature(s)aSource or reference
Escherichia coli
    XL1-BlueCloning strain; Tetr; ANG127Stratagene
    BL21(DE3)Strain for overproduction of proteins; ANG191Novagen
    T7IQStrain for overproduction of proteins; ANG2712NEB
    ANG1867pET28b in E. coli; KanrNovagen
    ANG2703BL21(DE3)/pET28b-kdpD-FL(3–885)-His Kanr21
    ANG2694XL1-Blue/pET28b-kdpD-NT(3–383)-His KanrThis study
    ANG2704BL21(DE3)/pET28b-kdpD-NT(3–383)-His KanrThis study
    ANG2697XL1-Blue/pET28b-kdpD-CT(492–885)-His KanrThis study
    ANG2707BL21(DE3)/pET28b-kdpD-CT(492–885)-His KanrThis study
    ANG3119XL1-Blue/pET28b-kdpD-KdpD(3–225)-His KanrThis study
    ANG3121BL21(DE3)/pET28b-kdpD-KdpD(3–225)-His KanrThis study
    ANG3120XL1-Blue/pET28b-kdpD-USP(225–383)-His KanrThis study
    ANG3122BL21(DE3)/pET28b-kdpD-USP(225–383)-His KanrThis study
    ANG3166XL1-Blue/pET28b-kdpD2-His KanrThis study
    ANG3167BL21(DE3)/pET28b-kdpD2-His KanrThis study
    ANG3152T7IQ/pVL847-Gn-GW-His-MBP-SACOL0066 Genr CamrThis study
    ANG3153T7IQ/pVL847-Gn-GW-His-MBP-SACOL00556 Genr CamrThis study
    ANG3154T71Q/pVL847-Gn-GW-His-MBP-SACOL1753 Genr CamrThis study
    ANG3155T7IQ/pVL847-Gn-GW-His-MBP-SACOL1759 Genr CamrThis study
    ANG3117XL1-Blue/pMALX(E) Ampr39
    ANG3175XL1-Blue/pMALX(E)-MBP-kdpD(USP) AmprThis study
    ANG3177BL21(DE3)/pMALX(E)-MBP-kdpD(USP) AmprThis study
    ANG3518XL1-Blue/pMALX(E)-MBP-kdpD(USP)S244A AmprThis study
    ANG3525BL21(DE3)/pMALX(E)-MBP-kdpD(USP)S244A AmprThis study
    ANG3520XL1-Blue/pMALX(E)-MBP-kdpD(USP)Y271A AmprThis study
    ANG3527BL21(DE3)/pMALX(E)-MBP-kdpD(USP)Y271A AmprThis study
Staphylococcus aureus
    MRSA252Wild-type MRSA strain; ANG311871
    LAC*Wild-type CA-MRSA strain (AH1263); ANG157572
    ANG1961LAC* gdpP::kan Kanr37
  • a The following antibiotics were used: 30 μg/ml kanamycin (Kan), 100 μg/ml ampicillin (Amp), 20 μg/ml gentamicin (Gen), and 10 μg/ml chloramphenicol (Cam) for E. coli cultures and 90 μg/ml kanamycin for S. aureus cultures. MRSA, methicillin-resistant Staphylococcus aureus; CA-MRSA, community-associated methicillin-resistant Staphylococcus aureus.